Konturek J W, Gabryelewicz A, Kulesza E, Konturek S J, Domschke W
Department of Medicine B, University of Münster, Germany.
Int J Pancreatol. 1995 Feb;17(1):55-61. doi: 10.1007/BF02788359.
Activation of type A receptors by CCK or cerulein is known to stimulate pancreatic enzyme secretion, but its role in the amino acid (AA) consumption and enzyme synthesis remains unclear. In our study, we used loxiglumide, a potent CCK-A-receptor antagonist, to investigate the role of CCK-A receptors in pancreatic consumption of circulating AAs and enzyme secretion. Five healthy male volunteers were intubated with double-lumen duodenal tube, and duodenal aspirates were collected during 60-min basal periods and then during pancreatic stimulation with iv infusion of secretion (80 pmol/kg/h) plus cerulein (50 pmol/kg/h) during three consecutive 30-min periods. The same procedure was repeated, but secretin-cerulein infusion was combined with a constant dose of loxiglumide (20 mumol/kg/h). The volume and outputs of HCO3-, protein and enzymes (amylase and trypsin) in duodenal aspirates and gallbladder volume (by sonography) were determined at 30-min intervals. Plasma samples were drawn for total plasma AA assay by ninhydrin method to assess the pancreatic uptake of free AAs. Infusion of secretin plus cerulein caused a several-fold increase in the volume of duodenal aspirate and the outputs of HCO3-, protein, and enzymes. During those periods, plasma AA level decreased from initially 2.20 +/- 0.3 mmol/L to 1.09 +/- 0.3 mmol/L (p < 0.01) and the gallbladder volume from initially 28 +/- 8 mL to 2 +/- 0.4 mL. This increase in pancreatic secretory outputs was accompanied by significant increments in plasma insulin, glucagon, PP, and somatostatin.(ABSTRACT TRUNCATED AT 250 WORDS)
已知胆囊收缩素（CCK）或雨蛙肽激活A 型受体可刺激胰腺酶分泌，但其在氨基酸（AA）消耗及酶合成中的作用仍不清楚。在我们的研究中，我们使用了强效CCK - A受体拮抗剂洛西格列肽，来研究CCK - A受体在胰腺对循环中氨基酸的消耗及酶分泌中的作用。五名健康男性志愿者经双腔十二指肠管插管，在60分钟的基础期收集十二指肠抽吸物，然后在连续三个30分钟期间静脉输注促胰液素（80 pmol/kg/h）加雨蛙肽（50 pmol/kg/h）刺激胰腺时收集。重复相同程序，但促胰液素 - 雨蛙肽输注与恒定剂量的洛西格列肽（20 μmol/kg/h）联合使用。每隔30分钟测定十二指肠抽吸物中HCO3 - 、蛋白质和酶（淀粉酶和胰蛋白酶）的体积和输出量以及胆囊体积（通过超声检查）。采集血浆样本，采用茚三酮法进行总血浆氨基酸测定，以评估胰腺对游离氨基酸的摄取。输注促胰液素加雨蛙肽使十二指肠抽吸物的体积以及HCO3 - 、蛋白质和酶的输出量增加了几倍。在这些期间，血浆氨基酸水平从最初的2.20±0.3 mmol/L降至1.09±0.3 mmol/L（p<0.01），胆囊体积从最初的28±8 mL降至2±0.4 mL。胰腺分泌输出的这种增加伴随着血浆胰岛素、胰高血糖素、PP和生长抑素的显著增加。（摘要截断于250字）
