α-甲基甲硫氨酸对大肠杆菌生长及高丝氨酸O-转琥珀酰酶的抑制作用。
Inhibition of growth of Escherichia coli and of homoserine O-transsuccinylase by alpha-methylmethionine.
作者信息
Schlesinger S
出版信息
J Bacteriol. 1967 Aug;94(2):327-32. doi: 10.1128/jb.94.2.327-332.1967.
Abstract
The methionine analogue, alpha-methylmethionine, inhibits bacterial growth, but its action is overcome by methionine, homocysteine, and cystathionine. The effect of the analogue on growth is attributed to its ability to mimic methionine as a feed-back inhibitor of the first enzyme specific to methionine biosynthesis. This conclusion is based on the findings that (i) alpha-methylmethionine inhibits excretion of O-succinylhomoserine, the product of the first enzyme, by a methionine auxotroph unable to convert succinylhomoserine to cystahionine, and that (ii) the enzyme homoserine O-transsuccinylase is inhibited by alpha-methylmethionine in extracts of Escherichia coli. alpha-Methylmethionine also inhibits methionyl-ribonucleic acid synthetase in extracts, but this inhibition probably does not affect growth.
摘要
甲硫氨酸类似物α-甲基甲硫氨酸可抑制细菌生长，但其作用可被甲硫氨酸、同型半胱氨酸和胱硫醚克服。该类似物对生长的影响归因于其能够模拟甲硫氨酸，作为甲硫氨酸生物合成中第一种酶的反馈抑制剂。这一结论基于以下发现：（i）α-甲基甲硫氨酸抑制了一种无法将琥珀酰高丝氨酸转化为胱硫醚的甲硫氨酸营养缺陷型菌株对第一种酶的产物O-琥珀酰高丝氨酸的排泄；（ii）在大肠杆菌提取物中，高丝氨酸O-转琥珀酰酶受到α-甲基甲硫氨酸的抑制。α-甲基甲硫氨酸还抑制提取物中的甲硫氨酰核糖核酸合成酶，但这种抑制可能不会影响生长。
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