Kline B C, Schoenhard D E
J Bacteriol. 1969 Oct;100(1):365-9. doi: 10.1128/jb.100.1.365-369.1969.
Sulfate-utilizing revertants of a cysteine auxotroph of Salmonella pullorum have been found that cause an accumulation in growth medium of a sulfur compound more oxidized than sulfide. The sulfur compound was presumptively identified as sulfite by the formation of a colored complex in the presence of basic fuchsin and formaldehyde, oxidation to sulfate by peroxide, and acid-volatility. The acid-volatile material was identified as sulfite by formation of an S-sulfonyl derivative of 5,5'-dithio-bis(2-nitrobenzoic acid) which was chromatographically and electrophoretically identical to an authentic S-sulfonyl derivative. The presence of sulfate in minimal medium is required for sulfite accumulation, and both cysteine and selenate inhibit the accumulation. No evidence was obtained to indicate that a reduced sulfur compound was the precursor of the accumulated sulfite.
已发现鸡白痢沙门氏菌半胱氨酸营养缺陷型的硫酸盐利用回复突变体，其在生长培养基中会积累一种比硫化物氧化程度更高的硫化合物。通过在碱性品红和甲醛存在下形成有色络合物、用过氧化物氧化成硫酸盐以及酸挥发性，初步确定该硫化合物为亚硫酸盐。通过形成5,5'-二硫代双(2-硝基苯甲酸)的S-磺酰基衍生物，将酸挥发性物质鉴定为亚硫酸盐，该衍生物在色谱和电泳上与 authentic S-磺酰基衍生物相同。亚硫酸盐积累需要在基本培养基中存在硫酸盐，半胱氨酸和硒酸盐均抑制积累。未获得证据表明还原态硫化合物是积累的亚硫酸盐的前体。
