Kawabata N
Microbiol Immunol. 1980;24(4):271-9. doi: 10.1111/j.1348-0421.1980.tb02830.x.
Peptone-yeast extract (PY) medium containing 0.035% ferric ammonium citrate as an indicator, 0.05% sulfite as a substrate, 0.05% cysteine as a reducer and 0.5% glucose was found to be suitable for observing the sulfite reduction test. The effect of added cysteine on the test was suppressed by the addition of glucose. In cultures of bacteria grown for 2 days at 37 C in medium containing the above ingredients, 121 among 132 strains of clostridia, including 86 strains of Clostridium perfringens, gave a positive reaction. Although some strains of Salmonella and Proteus were positive, the specificity of the test for clostridia was thought to be relatively high. Positive reactions in a resting cell system were limited to some species of clostridia.
含有0.035%柠檬酸铁铵作为指示剂、0.05%亚硫酸盐作为底物、0.05%半胱氨酸作为还原剂和0.5%葡萄糖的蛋白胨酵母提取物（PY）培养基被发现适用于观察亚硫酸盐还原试验。添加葡萄糖会抑制添加半胱氨酸对试验的影响。在含有上述成分的培养基中于37℃培养2天的细菌培养物中，132株梭菌中的121株，包括86株产气荚膜梭菌，呈阳性反应。虽然一些沙门氏菌和变形杆菌菌株呈阳性，但该试验对梭菌的特异性被认为相对较高。静止细胞系统中的阳性反应仅限于某些梭菌种类。
