一种快速分离脑核糖核酸的改进方法。
An improved method for the rapid isolation of brain ribonucleic acid.
作者信息
Kaplan B B, Bernstein S L, Gioio A E
出版信息
Biochem J. 1979 Oct 1;183(1):181-4. doi: 10.1042/bj1830181.
Abstract
An improved one-step method for the extraction of RNA from rat brain is described. Fresh or frozen tissue is disrupted in the powerful protein denaturant guanidine thiocyanate, and RNA isolated by ultracentrifugation through CsCl. The procedure is advantageous in that it is relatively simple, is rapid and does not expose the sample to enzyme treatments or repeated organic extractions.
摘要
本文描述了一种改进的从大鼠脑提取RNA的一步法。新鲜或冷冻组织在强蛋白变性剂硫氰酸胍中匀浆，然后通过氯化铯超速离心分离RNA。该方法的优点是相对简单、快速，且样品无需进行酶处理或反复的有机抽提。
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