乙酸铵在核糖核酸沉淀中的应用。
The use of ammonium acetate in the precipitation of ribonucleic acid.
作者信息
Osterburg H H, Allen J K, Finch C E
出版信息
Biochem J. 1975 May;147(2):367-8. doi: 10.1042/bj1470367.
Abstract
Ammonium acetate (0.24M) in combination with cold 66% (v/v) ethanol quantitatively precipitated RNA from very dilute solutions (greater than or equal to mug/ml) after centrifugation for 2.5 X 10(6)g-min. There was also less co-precipitation of detergents with ammonium acetate than NaCl.
摘要
0.24M乙酸铵与冷的66%（v/v）乙醇混合，在2.5×10⁶g·min离心后，能从极稀溶液（≥微克/毫升）中定量沉淀RNA。与氯化钠相比，乙酸铵与去污剂的共沉淀也更少。
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