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1
The use of ammonium acetate in the precipitation of ribonucleic acid.乙酸铵在核糖核酸沉淀中的应用。
Biochem J. 1975 May;147(2):367-8. doi: 10.1042/bj1470367.
2
RNA purification--precipitation methods.RNA纯化——沉淀方法。
Methods Enzymol. 2013;530:337-43. doi: 10.1016/B978-0-12-420037-1.00019-1.
3
Selective precipitation of ribonucleic acid from a mixture of total cellular nucleic acids extracted from cultured mammalian cells.从培养的哺乳动物细胞中提取的总细胞核酸混合物中选择性沉淀核糖核酸。
Biochem J. 1971 Jan;121(1):27-31. doi: 10.1042/bj1210027.
4
Ammonium removal from aqueous solutions by zeolite adsorption together with chemical precipitation.沸石吸附与化学沉淀法去除水溶液中的铵。
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5
Rapid chromatographic separation of ribosomal ribonucleic acids.核糖体核糖核酸的快速色谱分离
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6
Optimalisation of magnesium ammonium phosphate precipitation and its applicability to the removal of ammonium.磷酸镁铵沉淀的优化及其在铵去除中的应用
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Quantitative precipitation of short oligonucleotides with low concentrations of cetyltrimethylammonium bromide.低浓度十六烷基三甲基溴化铵对短寡核苷酸的定量沉淀
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Ammonium sulfate precipitation of the glucocorticoid receptor from rat liver.大鼠肝脏糖皮质激素受体的硫酸铵沉淀法
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Optimization of the ethanol precipitation of RNA from formamide containing solutions.从含甲酰胺溶液中进行RNA乙醇沉淀的优化。
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The use of ammonium sulphate globulin precipitation for determination of affinity of anti-protein antibodies in mouse serum.使用硫酸铵球蛋白沉淀法测定小鼠血清中抗蛋白抗体的亲和力。
Immunology. 1972 May;22(5):747-56.

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Nuclear matrix and hnRNP share a common structural constituent associated with premessenger RNA.核基质和不均一核糖核蛋白体共享一种与信使前体RNA相关的共同结构成分。
EMBO J. 1983;2(6):953-60. doi: 10.1002/j.1460-2075.1983.tb01527.x.
4
Translation of tyrosine hydroxylase from poly(A)-mRNA in pheochromocytoma cells is enhanced by dexamethasone.在嗜铬细胞瘤细胞中,地塞米松可增强酪氨酸羟化酶从多聚腺苷酸信使核糖核酸(poly(A)-mRNA)的翻译。
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Translational activity of messenger ribonucleic acid isolated from unstimulated and phytohaemagglutinin-activated lymphocytes.从未经刺激和经植物血凝素激活的淋巴细胞中分离出的信使核糖核酸的翻译活性。
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9
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10
Isolation of messenger RNA coding for the "fast" protein of embryonic chick feathers.编码鸡胚胎羽毛“快速”蛋白的信使核糖核酸的分离
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本文引用的文献

1
Genomic function during the lampbrush chromosome stage of amphibian oogenesis.两栖类卵子发生灯刷染色体阶段的基因组功能。
Proc Natl Acad Sci U S A. 1966 Sep;56(3):856-63. doi: 10.1073/pnas.56.3.856.
2
ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID.核糖体核糖核酸的分离与鉴定
Biochem J. 1965 Jul;96(1):266-9. doi: 10.1042/bj0960266.
3
Persistence in early amphibian embryos of informational RNA's from the lampbrush chromosome stage of oögenesis.卵母细胞灯刷染色体阶段的信息RNA在早期两栖类胚胎中的存留情况。
Proc Natl Acad Sci U S A. 1967 Apr;57(4):885-92. doi: 10.1073/pnas.57.4.885.
4
A procedure for the isolation of mammalian messenger ribonucleic acid.一种分离哺乳动物信使核糖核酸的方法。
Biochemistry. 1972 Feb 15;11(4):637-41. doi: 10.1021/bi00754a027.
5
General interspersion of repetitive with non-repetitive sequence elements in the DNA of Xenopus.非洲爪蟾DNA中重复序列元件与非重复序列元件的总体散布情况。
J Mol Biol. 1973 Jun 15;77(1):1-23. doi: 10.1016/0022-2836(73)90359-8.

乙酸铵在核糖核酸沉淀中的应用。

The use of ammonium acetate in the precipitation of ribonucleic acid.

作者信息

Osterburg H H, Allen J K, Finch C E

出版信息

Biochem J. 1975 May;147(2):367-8. doi: 10.1042/bj1470367.

DOI:10.1042/bj1470367
PMID:1180897
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165452/
Abstract

Ammonium acetate (0.24M) in combination with cold 66% (v/v) ethanol quantitatively precipitated RNA from very dilute solutions (greater than or equal to mug/ml) after centrifugation for 2.5 X 10(6)g-min. There was also less co-precipitation of detergents with ammonium acetate than NaCl.

摘要

0.24M乙酸铵与冷的66%(v/v)乙醇混合,在2.5×10⁶g·min离心后,能从极稀溶液(≥微克/毫升)中定量沉淀RNA。与氯化钠相比,乙酸铵与去污剂的共沉淀也更少。