通过制备性聚丙烯酰胺凝胶电泳纯化低分子量金属结合蛋白：电泳纯化的大鼠肝脏（镉、锌）-金属硫蛋白的性质
Purification of low molecular weight metal-binding proteins by preparative polyacrylamide gel electrophoresis: properties of electrophoretically purified rat liver (Cd, Zn) - metallothioneins.
作者信息
Zelazowski A J, Szymańska J A, Witas H W
出版信息
Prep Biochem. 1980;10(4):495-505. doi: 10.1080/00327488008061746.
PMID:7413609
Abstract
A method is proposed for purification of metallothionein by preparative polyacrylamide gel electrophoresis. The method enables purification of 100-700 mg of a preparation containing (Cd, Zn) - metallothionein yielding preparations of considerably higher purity as compared with those obtained by ion-exchange chromatography.
摘要
提出了一种通过制备型聚丙烯酰胺凝胶电泳纯化金属硫蛋白的方法。该方法能够纯化100 - 700毫克含有（镉、锌）-金属硫蛋白的制剂，与通过离子交换色谱法获得的制剂相比，所得制剂的纯度要高得多。
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