不同植物颗粒组分中叶绿素a的吸收与荧光特性
Absorption and fluorescence of chlorophyll a in particle fractions from different plants.
作者信息
Brown J S
出版信息
Biophys J. 1969 Dec;9(12):1542-52. doi: 10.1016/S0006-3495(69)86469-6.
Abstract
Density-gradient centrifugation of disintegrated cells from a variety of plants gave two kinds of chlorophyll particles from all except the blue-green algae. As in previous procedures using detergents, the lighter Fraction 1 particles usually had greater absorption at longer wavelengths; they always had a lower ratio of short to long wavelength fluorescence at low temperature, and a lower fluorescence yield per chlorophyll than the denser Fraction 2 particles. Although only one kind of particle fraction was found in each blue-green alga, the fine structure of the chlorophyll a absorption band differed significantly among the three species measured.
摘要
对来自多种植物的破碎细胞进行密度梯度离心，除蓝藻外，其他植物均得到了两种叶绿素颗粒。与之前使用去污剂的方法一样，较轻的1号组分颗粒通常在较长波长处有更大的吸收；在低温下，它们的短波长与长波长荧光比值总是较低，且每单位叶绿素的荧光产率也比密度较大的2号组分颗粒低。虽然在每种蓝藻中只发现了一种颗粒组分，但在所测定的三个物种中，叶绿素a吸收带的精细结构存在显著差异。
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