绵羊肝脏6-磷酸葡萄糖酸脱氢酶的纯化及性质
Purification and properties of 6-phosphogluconate dehydrogenase from sheep liver.
作者信息
Villet R H, Dalziel K
出版信息
Biochem J. 1969 Dec;115(4):639-43. doi: 10.1042/bj1150639.
Abstract
A method is described for the isolation of 6-phosphogluconate dehydrogenase from sheep liver. The product appears to be homogeneous in polyacrylamide-gel electrophoresis and in sedimentation-velocity and sedimentation-equilibrium studies in the ultracentrifuge. The molecular weight is estimated as 129000 from equilibrium sedimentation.
摘要
本文描述了一种从羊肝中分离6-磷酸葡萄糖酸脱氢酶的方法。该产物在聚丙烯酰胺凝胶电泳以及超速离心机中的沉降速度和沉降平衡研究中似乎是均一的。通过平衡沉降法估计其分子量为129000。
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