• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用各种固定化三嗪亲和染料从嗜热脂肪芽孢杆菌中纯化6-磷酸葡萄糖酸脱氢酶。

The use of various immobilized-triazine affinity dyes for the purification of 6-phosphogluconate dehydrogenase from Bacillus stearothermophilus.

作者信息

Qadri F, Dean P D

出版信息

Biochem J. 1980 Oct 1;191(1):53-62. doi: 10.1042/bj1910053.

DOI:10.1042/bj1910053
PMID:7470099
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1162181/
Abstract
  1. 6-Phosphogluconate dehydrogenase from Bacillus stearothermophilus was purified approximately 260-fold on triazine-immobilized dye columns to a final specific activity of 54 mumol of NADP+ reduced/min per mg of protein and an overall yield of 62%. 2. An investigation of the capacities of different triazine dyes that inhibit 6-phosphogluconate dehydrogenase was carried out. Cibacron Blue F3G-A and Procion Red HE-3B strongly inhibited the enzyme in free solution and were therefore chosen as the ligands in the purification scheme. 3. KCl was found to be the most suitable agent for eluting 6-phosphogluconate dehydrogenase from Procion Red HE-3B-Sepharose 6B. NADP+ could specifically elute 6-phosphogluconate dehydrogenase from Cibacron Blue F3G-A-Sepharose 6B. 4. A study of the effect of temperature on the binding of pure 6-phosphogluconate dehydrogenase to both Cibacron Blue-Sepharose and Procion Red-Sepharose showed that the binding increased with an increase in temperature.
摘要
  1. 嗜热脂肪芽孢杆菌的6-磷酸葡萄糖酸脱氢酶在三嗪固定化染料柱上纯化了约260倍，最终比活性为每毫克蛋白质每分钟还原54微摩尔NADP⁺，总产率为62%。2. 对不同抑制6-磷酸葡萄糖酸脱氢酶的三嗪染料的能力进行了研究。汽巴克隆蓝F3G-A和普施安红HE-3B在游离溶液中强烈抑制该酶，因此被选作纯化方案中的配体。3. 发现KCl是从普施安红HE-3B-琼脂糖6B上洗脱6-磷酸葡萄糖酸脱氢酶的最合适试剂。NADP⁺可从汽巴克隆蓝F3G-A-琼脂糖6B上特异性洗脱6-磷酸葡萄糖酸脱氢酶。4. 对温度对纯6-磷酸葡萄糖酸脱氢酶与汽巴克隆蓝-琼脂糖和普施安红-琼脂糖结合的影响的研究表明，结合随温度升高而增加。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e51/1162181/51af2374c644/biochemj00414-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e51/1162181/51af2374c644/biochemj00414-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e51/1162181/51af2374c644/biochemj00414-0064-a.jpg

相似文献

1
The use of various immobilized-triazine affinity dyes for the purification of 6-phosphogluconate dehydrogenase from Bacillus stearothermophilus.使用各种固定化三嗪亲和染料从嗜热脂肪芽孢杆菌中纯化6-磷酸葡萄糖酸脱氢酶。
Biochem J. 1980 Oct 1;191(1):53-62. doi: 10.1042/bj1910053.
2
Interactions of immobilized and free triazine dyes with glucose-6-phosphate dehydrogenase from yeast.固定化和游离三嗪染料与酵母葡萄糖-6-磷酸脱氢酶的相互作用。
Biomed Biochim Acta. 1986;45(3):273-80.
3
The effect of nucleotide presaturation on the chromatographic behaviour of 6-phosphogluconate dehydrogenase from Bacillus stearothermophilus on immobilized triazine dyes.核苷酸预饱和对嗜热脂肪芽孢杆菌6-磷酸葡萄糖酸脱氢酶在固定化三嗪染料上色谱行为的影响。
Biochem J. 1981 Dec 1;199(3):471-2. doi: 10.1042/bj1990471.
4
The selective retardation of NADP+-dependent dehydrogenases by immobilized procion red HE-3B.固定化普施安红HE - 3B对NADP + 依赖性脱氢酶的选择性抑制作用
Biochem J. 1978 Aug 1;173(2):591-6. doi: 10.1042/bj1730591.
5
6-Phosphogluconate dehydrogenase from Bacillus stearothermophilus.嗜热脂肪芽孢杆菌的6-磷酸葡萄糖酸脱氢酶。
Methods Enzymol. 1982;89 Pt D:282-91. doi: 10.1016/s0076-6879(82)89051-4.
6
The effect of ligand presaturation on the interaction of serum albumins with an immobilized Cibacron Blue 3G-A studied by affinity gel electrophoresis.通过亲和凝胶电泳研究配体预饱和对血清白蛋白与固定化汽巴蓝3G - A相互作用的影响。
Biochem J. 1981 Dec 1;199(3):465-72. doi: 10.1042/bj1990465.
7
Interaction of bacterial glucose-6-phosphate dehydrogenase with triazine dyes: a study by means of affinity partitioning and kinetic analysis.细菌葡萄糖-6-磷酸脱氢酶与三嗪染料的相互作用：亲和分配及动力学分析研究
Biomed Biochim Acta. 1990;49(4):151-60.
8
The application of triazine dye affinity chromatography to the large-scale purification of glycerokinase from Bacillus stearothermophilus.三嗪染料亲和色谱法在嗜热脂肪芽孢杆菌甘油激酶大规模纯化中的应用。
Anal Biochem. 1983 Jul 15;132(2):413-7. doi: 10.1016/0003-2697(83)90028-3.
9
[A comparative study of the interaction between glutathione reductase and 6-phosphogluconate dehydrogenase of rat liver with certain affinity sorbents].[大鼠肝脏谷胱甘肽还原酶与6-磷酸葡萄糖酸脱氢酶与某些亲和吸附剂相互作用的比较研究]
Ukr Biokhim Zh (1978). 1994 Jul-Aug;66(4):58-65.
10
Affinity labelling of enzymes with triazine dyes. Isolation of a peptide in the catalytic domain of horse-liver alcohol dehydrogenase using Procion blue MX-R as a structural probe.用三嗪染料对酶进行亲和标记。使用普施安蓝MX-R作为结构探针分离马肝醇脱氢酶催化结构域中的一个肽段。
Eur J Biochem. 1982 Nov;128(1):119-23.

引用本文的文献

1
The effect of nucleotide presaturation on the chromatographic behaviour of 6-phosphogluconate dehydrogenase from Bacillus stearothermophilus on immobilized triazine dyes.核苷酸预饱和对嗜热脂肪芽孢杆菌6-磷酸葡萄糖酸脱氢酶在固定化三嗪染料上色谱行为的影响。
Biochem J. 1981 Dec 1;199(3):471-2. doi: 10.1042/bj1990471.
2
Simultaneous purification and characterization of glucokinase, fructokinase and glucose-6-phosphate dehydrogenase from Zymomonas mobilis.从运动发酵单胞菌中同时纯化和鉴定葡萄糖激酶、果糖激酶和葡萄糖-6-磷酸脱氢酶。
Biochem J. 1985 Jun 15;228(3):627-34. doi: 10.1042/bj2280627.

本文引用的文献

1
DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.圆盘电泳。一、背景与理论。
Ann N Y Acad Sci. 1964 Dec 28;121:321-49. doi: 10.1111/j.1749-6632.1964.tb14207.x.
2
Affinity gel filtration: A new method for the rapid determination of apparent molecular weights of enzymes.亲和凝胶过滤：一种快速测定酶表观分子量的新方法。
FEBS Lett. 1971 Oct 15;18(1):31-34. doi: 10.1016/0014-5793(71)80399-x.
3
Studies on the mechanism of binding of serum albumins to immobilized cibacron blue F3G A.血清白蛋白与固定化汽巴蓝F3G A结合机制的研究。
Biochem J. 1980 Jul 1;189(1):27-34. doi: 10.1042/bj1890027.
4
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
5
Purification and properties of 6-phosphogluconate dehydrogenase from sheep liver.绵羊肝脏6-磷酸葡萄糖酸脱氢酶的纯化及性质
Biochem J. 1969 Dec;115(4):639-43. doi: 10.1042/bj1150639.
6
6-Phosphogluconate dehydrogenase from Bacillus stearothermophilus.嗜热脂肪芽孢杆菌的6-磷酸葡萄糖酸脱氢酶。
FEBS Lett. 1973 Dec 15;38(1):49-52. doi: 10.1016/0014-5793(73)80510-1.
7
Affinity chromatography on immobilised adenosine 5'-monophosphate. 5. Some applications of the influence of temperature on the binding of dehydrogenases and kinases.固定化5'-单磷酸腺苷亲和层析。5. 温度对脱氢酶和激酶结合影响的一些应用
Eur J Biochem. 1974 Jan 16;41(2):353-7. doi: 10.1111/j.1432-1033.1974.tb03277.x.
8
Applications of blue dextran and Cibacron Blue F3GA in purification and structural studies of nucleotide-requiring enzymes.蓝色葡聚糖和汽巴蓝F3GA在需要核苷酸的酶的纯化及结构研究中的应用。
Biochem Biophys Res Commun. 1976 Oct 4;72(3):816-23. doi: 10.1016/s0006-291x(76)80206-9.
9
X-ray studies of the binding of Cibacron blue F3GA to liver alcohol dehydrogenase.汽巴克隆蓝F3GA与肝脏乙醇脱氢酶结合的X射线研究。
Eur J Biochem. 1979 Dec;102(1):107-10. doi: 10.1111/j.1432-1033.1979.tb06268.x.
10
Protein purification using immobilised triazine dyes.使用固定化三嗪染料进行蛋白质纯化。
J Chromatogr. 1979 Oct 1;165(3):301-19. doi: 10.1016/s0021-9673(00)88187-x.