猫体内[14C]尼古丁的代谢
The metabolism of [14C] nicotine in the cat.
作者信息
Turner D M
出版信息
Biochem J. 1969 Dec;115(5):889-96. doi: 10.1042/bj1150889.
The metabolism of [2'-(14)C]nicotine given as an intravenous injection in small doses to anaesthetized and unanaesthetized cats has been studied. A method is described for the quantitative determination of [(14)C]nicotine and [(14)C]cotinine in tissues and body fluids. Nanogram amounts of these compounds have been detected. After a single dose of 40mug. of [(14)C]nicotine/kg., 55% of the injected radioactivity was excreted in the urine within 24hr., but only 1% of this radioactivity was unchanged nicotine. [(14)C]Nicotine is metabolized extremely rapidly, [(14)C]cotinine appearing in the blood within 2.5min. of intravenous injection. [(14)C]Nicotine accumulates rapidly in the brain and 15min. after injection 90% of the radioactivity still represents [(14)C]nicotine. Metabolites of [(14)C]nicotine have been identified in liver and urine extracts. [(14)C]Nicotine-1'-oxide has been detected in both liver and urine.
研究了小剂量静脉注射[2'-(14)C]尼古丁后，麻醉和未麻醉猫体内的代谢情况。描述了一种定量测定组织和体液中[(14)C]尼古丁和[(14)C]可替宁的方法。已检测到纳克量的这些化合物。单次给予40微克[(14)C]尼古丁/千克后，24小时内55%的注入放射性物质经尿液排出，但其中只有1%的放射性物质是未变化的尼古丁。[(14)C]尼古丁代谢极快，静脉注射后2.5分钟内血液中就出现[(14)C]可替宁。[(14)C]尼古丁在大脑中迅速蓄积，注射后15分钟，90%的放射性物质仍为[(14)C]尼古丁。已在肝脏和尿液提取物中鉴定出[(14)C]尼古丁的代谢产物。在肝脏和尿液中均检测到[(14)C]尼古丁-1'-氧化物。
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