Schnelder de Zajol J M, Delost P
Reprod Nutr Dev (1980). 1981;21(5A):621-7.
We have estimated two parameters of guinea-pig adrenal function during the neonatal period: --the concentration of plasma cortisol by competitive binding to protein; --the association constant, Ka, and total capacity of CBG binding (NS) by the thin-layer gel filtration technique. The concentration of plasma cortisol, very high at birth, declined markedly between neonatal days 1 and 10 and then remained constant between days 10 and 20, the time of weaning. Ka was 0.25.10(7).M-1 in the newborn and the adult of both sexes. This value was lower than that of other species. NS, which was very high at birth (111 +/- 5 micrograms/100 ml in the male; 101 +/- 5 micrograms/100 ml in the female), decreased rapidly during the first two neonatal days. At that time, it reached adult levels in the female (45 +/- 5 micrograms/100 ml) and remained stable. Adult binding capacity (16 +/- 2 micrograms/100 ml) was reached in the male after neonatal day 20.
——通过与蛋白质竞争性结合来测定血浆皮质醇浓度；——采用薄层凝胶过滤技术测定皮质类固醇结合球蛋白（CBG）结合的结合常数Ka和总容量（NS）。血浆皮质醇浓度在出生时非常高，在出生后第1天到第10天之间显著下降，然后在第10天到第20天（断奶期）保持恒定。Ka在新生豚鼠及成年雌雄豚鼠中均为0.25×10⁷ M⁻¹。该值低于其他物种。NS在出生时非常高（雄性为111±5微克/100毫升；雌性为101±5微克/100毫升），在出生后的头两天迅速下降。此时，雌性豚鼠的NS达到成年水平（45±5微克/100毫升）并保持稳定。雄性豚鼠在出生后第20天达到成年结合能力（16±2微克/100毫升）。
