Bacchi C J, Vergara C, Garofalo J, Lipschik G Y, Hutner S H
J Protozool. 1979 Aug;26(3):484-8. doi: 10.1111/j.1550-7408.1979.tb04658.x.
The sensitive dansyl procedure was used to detect putrescine and spermidine, but not spermine and cadaverine, in pleomorphic Trypanosoma brucei. The polyamines were synthesized in vitro from [3H]ornithine, [14C]arginine and [14C]methionine. Proline, agmatine, and citrulline, but not glutamine, glutamic or pyroglutamic acids, stimulated spermidine formation from [4C]methionine. Putrescine and sperimidine synthesis occurred rapidly from ornithine: putrescine synthesis peaked in 0.5 h, spermidine in 1 h. Trypanosoma brucei assimilated exogenous 14C-labeled putrescine, spermidine, and spermine; spermidine and spermine were taken up 5 times as rapidly as putrescine. Polyamine syntheses may therefore be a practical target for novel trypanocies.
采用灵敏的丹磺酰法检测多形布氏锥虫中的腐胺和亚精胺，但未检测到精胺和尸胺。多胺由[3H]鸟氨酸、[14C]精氨酸和[14C]甲硫氨酸在体外合成。脯氨酸、胍丁胺和瓜氨酸，而非谷氨酰胺、谷氨酸或焦谷氨酸，刺激了[4C]甲硫氨酸生成亚精胺。鸟氨酸可快速合成腐胺和亚精胺：腐胺合成在0.5小时达到峰值，亚精胺在1小时达到峰值。布氏锥虫吸收外源性14C标记的腐胺、亚精胺和精胺；亚精胺和精胺的吸收速度是腐胺的5倍。因此，多胺合成可能是新型锥虫药物的一个实际靶点。
