Casida L E
Appl Microbiol. 1969 Dec;18(6):1065-71. doi: 10.1128/am.18.6.1065-1071.1969.
A procedure is described for visually observing and following the activities and interactions of bacteria, actinomyctes, fungi, protozoa, nematodes, and plant roots in masses of soil. Specific microscope components and objectives are used, and the numerical apertures are adjusted such that light diffraction colors are produced to allow differentiation of the various biological entities and their habitat materials. Strains or other alterations in the organisms and their habitat are not employed, and time-lapse photography can be used to follow the activities of soil microorganisms and plant roots. As a result of the use of this technique, it is apparent that in situ indigenous soil microorganisms differ from similar organisms grown in the laboratory, but that, under the proper conditions, the state of the organism in either habitat can be altered to match that which occurs in the contrasting habitat.
本文描述了一种用于直观观察和追踪土壤团块中细菌、放线菌、真菌、原生动物、线虫和植物根系的活动及相互作用的方法。使用了特定的显微镜组件和物镜，并调整数值孔径以产生光衍射颜色，从而能够区分各种生物实体及其栖息地物质。不采用对生物体及其栖息地进行菌株处理或其他改变的方法，并且可以使用延时摄影来追踪土壤微生物和植物根系的活动。使用该技术的结果表明，原位原生土壤微生物与在实验室中培养的类似生物不同，但是在适当条件下，任一栖息地中生物体的状态都可以改变，以与对比栖息地中发生的情况相匹配。
