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用于检测和研究土壤中微生物的连续可变振幅对比显微镜。

Continuously variable amplitude contrast microscopy for the detection and study of microorganisms in soil.

作者信息

Casida L E

出版信息

Appl Environ Microbiol. 1976 Apr;31(4):605-8. doi: 10.1128/aem.31.4.605-608.1976.

DOI:10.1128/aem.31.4.605-608.1976
PMID:773307
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC169825/
Abstract

A new type of phase microscope was used to detect and observe the microflora in soil and to differentiate cells and spores from soil debris. This microscope provides a continuous variation in the amplitude ratios between undeviated and deviated beams of light, and the microbial cells, spores, and debris in soil change in appearance to a differing degree in response to the changes in amplitude ratio.

摘要

一种新型相显微镜被用于检测和观察土壤中的微生物群落,并区分细胞和孢子与土壤碎片。这种显微镜能使未偏离和偏离光束之间的振幅比持续变化,土壤中的微生物细胞、孢子和碎片会随着振幅比的变化而在外观上产生不同程度的变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35dd/169825/ca2063c804ac/aem00004-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35dd/169825/ca2063c804ac/aem00004-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35dd/169825/ca2063c804ac/aem00004-0165-a.jpg

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本文引用的文献

1
USE OF THE MICROSCOPE IN STUDYING THE ACTIVITIES OF BACTERIA IN SOIL.显微镜在研究土壤中细菌活动中的应用。
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A comparison of a direct- and a plate counting technique for the quantitative estimation of soil micro-organisms.用于土壤微生物定量估计的直接计数法与平板计数法的比较。
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Observation of microorganisms in soil and other natural habitats.土壤及其他自然栖息地中微生物的观察。
Appl Microbiol. 1969 Dec;18(6):1065-71. doi: 10.1128/am.18.6.1065-1071.1969.
6
The use of fluorescein isothiocyanate in the determination of the bacterial biomass of grassland soil.异硫氰酸荧光素在草地土壤细菌生物量测定中的应用。
Can J Microbiol. 1970 Feb;16(2):57-62. doi: 10.1139/m70-011.
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Infrared color photography: selective demonstration of bacteria.
Science. 1968 Jan 12;159(3811):199-200. doi: 10.1126/science.159.3811.199.
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Microorganisms in soil as observed by staining with rhodamine-labeled lysozyme.
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Direct fluorescent labeling of microorganisms as a possible life-detection technique.将微生物直接荧光标记作为一种可能的生命探测技术。
Appl Microbiol. 1966 Jan;14(1):119-23. doi: 10.1128/am.14.1.119-123.1966.