Tonogai Y, Ito Y, Iwaida M, Tati M, Ose Y, Sato T
J Toxicol Sci. 1979 Aug;4(3):211-9. doi: 10.2131/jts.4.211.
The toxicity of xanthene dyes were studied by various interaction between the dyes and the components in vital body. (1) An increase in the amount of Rose Bengale adsorbed on the gill of fish was followed by the increase in red corpuscle number, and it was assumed that the death of fish in xanthene dye solution was due to anoxemia. (2) Binding capacity of xanthene dyes with bovine serum albumin decreased in the following; Rose Bengale, Phloxine, Erythrosine, Eosine and Uranine. This order was quite coincident with the toxicity compared by TLm values. (3) From the results of rec-assay test by use of Bacillus subtilis, it was confirmed that Phloxine and Rose Bengale had DNA-damaging capacity related to the mutagenecity.
通过研究呫吨染料与生物体内各成分之间的各种相互作用，对其毒性进行了研究。（1）吸附在鱼鳃上的孟加拉玫瑰红数量增加后，红细胞数量也随之增加，据推测，鱼类在呫吨染料溶液中的死亡是由于缺氧血症。（2）呫吨染料与牛血清白蛋白的结合能力按以下顺序降低：孟加拉玫瑰红、焰红染料、赤藓红、曙红和荧光素。这个顺序与通过半数耐受限量值比较的毒性完全一致。（3）通过使用枯草芽孢杆菌进行的重新测定试验结果证实，焰红染料和孟加拉玫瑰红具有与致突变性相关的DNA损伤能力。
