Matsushima Y, Gemba M
Jpn J Pharmacol. 1979 Jun;29(3):367-74. doi: 10.1254/jjp.29.367.
Correlative studies on transports of Ca2+ and Na+ and the properties of Ca2+ transport were carried out in rat kidney cortex slices. Ouabain had no effect on the Ca2+ transport but did inhibit the Na+ transport extensively. With addition of 2,4-dinitrophenol to the incubation medium, or under the anaerobic conditions, the effluxes of Ca2+ and Na+ were inhibited while the Ca2+ influx was enhanced significantly. Sulfhydryl inhibitors such as ethacrynic acid, mersalyl and p-chloromercuribenzoic acid suppressed Ca2+ efflux and stimulated Ca2+ influx. When the slices of kidney cortex were treated with these inhibitors, there was a reduction in the content of cellular ATP. The present results suggest that Ca2+ transport may be partly independent of Na+ transport, and that Ca2+ efflux may require energy-yielding processes.
在大鼠肾皮质切片中进行了钙（Ca2+）和钠（Na+）转运以及钙转运特性的相关研究。哇巴因对钙转运无影响，但能广泛抑制钠转运。向孵育培养基中添加2,4 -二硝基苯酚或在厌氧条件下，钙和钠的外流受到抑制，而钙内流显著增强。巯基抑制剂如依他尼酸、汞撒利和对氯汞苯甲酸可抑制钙外流并刺激钙内流。当用这些抑制剂处理肾皮质切片时，细胞ATP含量会降低。目前的结果表明，钙转运可能部分独立于钠转运，且钙外流可能需要产能过程。
