Kohda C, Gemba M
Jpn J Pharmacol. 1979 Oct;29(5):745-51. doi: 10.1254/jjp.29.745.
The effect of verapamil on Ca2+ and Mg2+ accumulation was investigated in isolated rat kidney cortex mitochondria. For the 50% inhibition of Ca2+ accumulation, 2 x 10(-4) M verapamil concentration was required in the presence of ATP (2 mM) and phosphate (5 mM). Omission of phosphate from the medium increased the inhibitory effect of verapamil on Ca2+ accumulation. Verapamil had no effect on Ca2+ accumulation in the presence of both ATP and succinate (7.8 mM), but further addition of phosphate resulted in a significant inhibition of Ca2+ accumulation by verapamil. Mg2+ accumulation of mitochondria was similarly depressed by verapamil. The same tendency was found as for the modification of verapamil effect by acetate in mitochondrial Ca2+ and Mg2+ accumulation. Succinate oxidation of mitochondria was not affected by verapamil in the absence of phosphate, but was inhibited by verapamil in the presence of phosphate. Therefore, it seemed reasonable to assume that the depression of Ca2+ and Mg2+ transport of mitochondria by verapamil is modulated by permeant anions.
在离体大鼠肾皮质线粒体中研究了维拉帕米对Ca2+和Mg2+积累的影响。在ATP（2 mM）和磷酸盐（5 mM）存在的情况下，抑制50%的Ca2+积累需要2×10(-4) M的维拉帕米浓度。培养基中省略磷酸盐会增加维拉帕米对Ca2+积累的抑制作用。在ATP和琥珀酸盐（7.8 mM）同时存在的情况下，维拉帕米对Ca2+积累没有影响，但进一步添加磷酸盐会导致维拉帕米对Ca2+积累的显著抑制。线粒体的Mg2+积累同样被维拉帕米抑制。在醋酸盐对线粒体Ca2+和Mg2+积累中维拉帕米作用的修饰方面也发现了相同的趋势。在没有磷酸盐的情况下，线粒体的琥珀酸氧化不受维拉帕米影响，但在有磷酸盐的情况下会被维拉帕米抑制。因此，似乎有理由假设，维拉帕米对线粒体Ca2+和Mg2+转运的抑制作用受渗透性阴离子调节。
