雌二醇受体和卵黄蛋白原基因在鸡肝脏染色质组分中的分布。
Distribution of estradiol receptor and vitellogenin gene in chick liver chromatin fractions.
作者信息
Alberga A, Tran A, Baulieu E E
出版信息
Nucleic Acids Res. 1979 Dec 11;7(7):2031-44. doi: 10.1093/nar/7.7.2031.
The distribution of estradiol receptor and vitellogenin gene was studied in estradiol stimulated chick liver chromatin fractions prepared by limited DNAse II digestion and MgCl2 precipitation. The receptor was found in all fractions, undigested chromatin (P1), Mg2+ insoluble chromatin (P2) and Mg2+ soluble chromatin (S2). This last fraction was rich in acidic proteins, had a high protein:DNA ratio (7.0 w/w), contained 28% of rapidly labelled RNA, 20% of the receptor, 3-5% of chromatin DNA and showed a 2 fold enrichment of vitellogenin DNA sequences over unfractionated chromatin as well as P1 and P2 DNA. On isopycnic metrizamide gradients, all chromatin fractions showed a receptor peak banding at 1.23 g/cm3, the density of nucleoproteins. Hybridization experiments showed that the DNA banding at this density in fraction S2 was enriched 4 fold in vitellogenin DNA sequences over unfractionated chromatin as well as P1 and P2 DNA. These results suggest an association of hormone receptor complex with nucleoprotein structures of an apparently active chromatin fraction.
采用有限的DNA酶II消化和氯化镁沉淀法制备雌二醇刺激的鸡肝染色质组分，研究了雌二醇受体和卵黄蛋白原基因的分布。在所有组分中均发现了受体，包括未消化的染色质（P1）、Mg2+不溶性染色质（P2）和Mg2+可溶性染色质（S2）。最后一个组分富含酸性蛋白，蛋白质与DNA的比例较高（7.0 w/w），含有28%的快速标记RNA、20%的受体、3 - 5%的染色质DNA，与未分级的染色质以及P1和P2 DNA相比，卵黄蛋白原DNA序列富集了2倍。在等密度的甲泛葡胺梯度上，所有染色质组分均显示受体峰在1.23 g/cm3处形成条带，此为核蛋白的密度。杂交实验表明，在组分S2中，该密度处的DNA条带与未分级的染色质以及P1和P2 DNA相比，卵黄蛋白原DNA序列富集了4倍。这些结果表明激素受体复合物与一个明显具有活性的染色质组分的核蛋白结构相关联。
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