在铬酸盐存在的情况下，各种物种红细胞中氧化型谷胱甘肽的转运。
The transport of oxidized glutathione from the erythrocytes of various species in the presence of chromate.
作者信息
Srivastava S K, Beutler E
出版信息
Biochem J. 1969 Oct;114(4):833-7. doi: 10.1042/bj1140833.
Abstract
- Erythrocytes from normal and glucose 6-phosphate dehydrogenase-deficient humans were subjected to hydrogen peroxide diffusion to oxidize the GSH. Studies were carried out in the presence and absence of chromate to inhibit glutathione reductase and with or without the addition of glucose. 2. The GSH content of erythrocytes from other species was oxidized by subjecting them to hydrogen peroxide diffusion in the presence of chromate and glucose. 3. Chromate (1.3mm) inhibited glutathione reductase by about 80%, whereas glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, hexokinase, phosphofructokinase and pyruvate kinase were not inhibited. 4. The GSSG formed was transported from the erythrocytes to the medium. 5. The transport rate of GSSG from glucose 6-phosphate dehydrogenase-deficient erythrocytes subjected to hydrogen peroxide diffusion in the presence of chromate was comparable with that from normal and glucose 6-phosphate dehydrogenase-deficient erythrocytes. 6. The rate of transport of GSSG from erythrocytes of various species studied could be ranked: pigeon>rabbit>rat>donkey>man>dog>horse>sheep>chicken>fish.
摘要
- 对正常人和葡萄糖-6-磷酸脱氢酶缺乏症患者的红细胞进行过氧化氢扩散处理，以氧化谷胱甘肽（GSH）。在有或没有铬酸盐存在的情况下进行研究，以抑制谷胱甘肽还原酶，并在添加或不添加葡萄糖的情况下进行。2. 通过在铬酸盐和葡萄糖存在下对其他物种的红细胞进行过氧化氢扩散，氧化其谷胱甘肽含量。3. 铬酸盐（1.3mmol/L）可抑制谷胱甘肽还原酶约80%，而葡萄糖-6-磷酸脱氢酶、6-磷酸葡萄糖酸脱氢酶、己糖激酶、磷酸果糖激酶和丙酮酸激酶未受抑制。4. 形成的氧化型谷胱甘肽（GSSG）从红细胞转运到培养基中。5. 在铬酸盐存在下，对葡萄糖-6-磷酸脱氢酶缺乏症患者的红细胞进行过氧化氢扩散处理后，GSSG的转运速率与正常人和葡萄糖-6-磷酸脱氢酶缺乏症患者的红细胞相当。6. 所研究的各种物种红细胞中GSSG的转运速率排序如下：鸽子＞兔子＞大鼠＞驴＞人＞狗＞马＞绵羊＞鸡＞鱼。
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