Beutler E
Science. 1969 Aug 8;165(3893):613-5. doi: 10.1126/science.165.3893.613.
Glutathione reductase of hemolyzates from clinically normal subjects is activated by the addition of flavin-adenine dinucleotide. One-half maximum stimulation could be achieved by approximately 0.02 micromolar flavin-adenine dinucleotide; prior addition of adenosine triphosphate, adenosine diphosphate, or adenosine mnonophosphate prevented activation. Stimulation of glutathione reductase activity of red cells of normal subjects occurred when they were given 5 milligrams of riboflavin daily for 8 days. The degree of stimulation in vitro by flavin-adenine dinucleotide and in vivo by riboflavin was inversely proportional to dietary intake of riboflavin. The variety of clinical disorders which have been associated with glutathione reductase deficiency may have, as a common denominator, abnormalities in flavin-adenine dinucleotide formation.
临床正常受试者溶血产物中的谷胱甘肽还原酶可通过添加黄素腺嘌呤二核苷酸来激活。约0.02微摩尔的黄素腺嘌呤二核苷酸可实现最大刺激的一半；预先添加三磷酸腺苷、二磷酸腺苷或一磷酸腺苷可阻止激活。正常受试者每日给予5毫克核黄素，持续8天，其红细胞谷胱甘肽还原酶活性会受到刺激。黄素腺嘌呤二核苷酸在体外的刺激程度和核黄素在体内的刺激程度与核黄素的饮食摄入量成反比。与谷胱甘肽还原酶缺乏相关的各种临床疾病可能有一个共同特征，即黄素腺嘌呤二核苷酸形成异常。
