Boyd A D, Spencer F C, Hirose H, Engelman R M, Cannon F D, Ferrebee J W, Rapaport F T
Transplant Proc. 1975 Dec;7(4):475-7.
Nine Cooperstown beagles of known DL-A genotypes were exposed to supralethal total-body irradiation and received bone-marrow allografts from DL-A-identical donors. Four to 5 months later, the resulting chimeras received orthotopic cardiac allografts from their corresponding donors of marrow. Six chimeras died of operative complications in the immediate postoperative period. The other 3 chimeras survived from 173 to 547 days; 1 dog died at 173 days as a result of right-sided heart failure, secondary to stenosis at the site of the pulmonary artery anastomosis. The other two recipients continue to be active and healthy at 545 and 547 days. The results indicate that dogs can be rendered specifically tolerant to orthotopic cardiac allografts by supralethal total-body irradiation and the transplantation of marrow obtained from the prospective allograft donor.
9只已知DL-A基因型的库珀斯敦小猎犬接受了超致死剂量的全身照射，并接受了来自DL-A相同供体的骨髓同种异体移植。4至5个月后，产生的嵌合体接受了来自相应骨髓供体的原位心脏同种异体移植。6只嵌合体在术后即刻死于手术并发症。其他3只嵌合体存活了173至547天；1只狗在173天时因肺动脉吻合部位狭窄继发右侧心力衰竭而死亡。另外两只受体在545天和547天时仍然活跃且健康。结果表明，通过超致死剂量的全身照射和从前瞻性同种异体移植供体获得的骨髓移植，狗可以对原位心脏同种异体移植产生特异性耐受。
