Rueter A, Schleicher J B
Appl Microbiol. 1969 Dec;18(6):1057-9. doi: 10.1128/am.18.6.1057-1059.1969.
Ethylene oxide (ETO) sterilization of polyvinyl trays used in an antiviral screening program was initiated to overcome seasonal outbreaks of bacterial and mycotic contamination of tissue culture cells. Trays sterilized by 100% ETO for 5 hr after 48 hr of prehumidification, or by 12% ETO plus 88% Freon 12 for 1, 3, 5 or 18 hr, followed by various methods of aeration, were seeded with several types of tissue culture cells and examined for contamination, toxicity, and monolayer quality. A 1-hr exposure in 12% ETO plus 88% Freon 12 was adequate for sterilization, although residual toxicity for tissue cultures remained. A 7-day aeration period at 37 C was sufficient to eliminate toxicity and allow the growth of good monolayers of WI-38, HEp-2 and primary bovine kidney cells. Sterilization with 100% ETO required 14 days of aeration at 37 C to eliminate cytotoxicity. Increased residual toxicity resulting from longer ETO sterilization periods required longer aeration times at 37 C or higher aeration temperatures for detoxification.
为克服组织培养细胞细菌和霉菌污染的季节性爆发，启动了用于抗病毒筛选项目的聚乙烯托盘的环氧乙烷（ETO）灭菌。将托盘在预加湿48小时后用100%环氧乙烷灭菌5小时，或用12%环氧乙烷加88%氟利昂12灭菌1、3、5或18小时，然后采用各种通气方法，接种几种类型的组织培养细胞，并检查污染情况、毒性和单层质量。在12%环氧乙烷加88%氟利昂12中暴露1小时足以灭菌，尽管对组织培养仍有残留毒性。在37℃下通气7天足以消除毒性，并使WI-38、HEp-2和原代牛肾细胞形成良好的单层生长。用100%环氧乙烷灭菌需要在37℃下通气14天以消除细胞毒性。环氧乙烷灭菌时间延长导致的残留毒性增加，需要在37℃下更长的通气时间或更高的通气温度来解毒。
