乙酸盐是分离出的菠菜叶绿体中长链脂肪酸合成的首选底物。
Acetate is the preferred substrate for long-chain fatty acid synthesis in isolated spinach chloroplasts.
作者信息
Roughan P G, Holland R, Slack C R
出版信息
Biochem J. 1979 Dec 15;184(3):565-9. doi: 10.1042/bj1840565.
Abstract
- Commercially available [2-14C]pyruvate and [2-14C]malonate were found to contain 3-6% (w/w) of [14C]acetate. 2. The contaminating [14C]acetate was efficiently utilized for fatty acid synthesis by isolated chloroplasts, whereas the parent materials were poorer substrates. 3. Maximum incorporation rates of the different substrates examined were (ng-atoms of C/h per mg of chlorophyll): [1-14C]acetate, 2676; [2-14C]pyruvate, 810; H14CO3-, 355; [2-14C]malonate, 19. 4. Products of CO2 fixation were probably not a significant carbon source for fatty acid synthesis in the presence of exogenous acetate.
摘要
- 发现市售的[2-¹⁴C]丙酮酸和[2-¹⁴C]丙二酸含有3-6%（w/w）的[¹⁴C]乙酸盐。2. 分离的叶绿体能够有效地利用污染的[¹⁴C]乙酸盐进行脂肪酸合成，而母体物质作为底物的效果较差。3. 所检测的不同底物的最大掺入率为（每毫克叶绿素每小时的碳纳摩尔数）：[1-¹⁴C]乙酸盐，2676；[2-¹⁴C]丙酮酸，810；H¹⁴CO₃⁻，355；[2-¹⁴C]丙二酸，19。4. 在存在外源乙酸盐的情况下，二氧化碳固定产物可能不是脂肪酸合成的重要碳源。
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