Ito Y, Bowman R L
Science. 1970 Jan 16;167(3916):281-3. doi: 10.1126/science.167.3916.281.
The liquid-liquid partition chromatographic system reported here involves a long helix of narrow-bore tubing. When the coiled tube is filled with one phase of a two-phase system and fed with the other phase, phase-interchange takes place in each turn of the coil, leaving a segment of the former phase as the stationary phase. Consequently, solutes present in either phase are subjected to a multistep partition process. The column efficiency, estimated on a separation of dinitrophenyl amino acids, is comparable to that of gas chromatography.
本文报道的液-液分配色谱系统包含一段长的窄孔螺旋管。当螺旋管中充满两相系统中的一相并通入另一相时，在螺旋管的每一圈都会发生相交换，留下一段前一相作为固定相。因此，存在于任一相中的溶质都会经历多步分配过程。基于二硝基苯基氨基酸的分离所估算的柱效与气相色谱相当。
