Ito Y, Bowman R L
Science. 1971 Jul 30;173(3995):420-2. doi: 10.1126/science.173.3995.420.
We have developed a new method of countercurrent chromatography which employs a vertical helical tube in the centrifugal field. The helical tube is arranged so that it does not rotate as it revolves, thus eliminating the need for rotating seals. When the gyrating tube is filled with either phase and the other phase is introduced into the tube in the proper direction, an equilibrium state results in which the two phases are split into multiple alternating segments within the coil. Each phase oscillates to and fro with the rotation as the moving phase is steadily eluted out through the other end of the tube. Consequently, solutes introduced into the tube are subjected to a rapid partition process, resulting in an efficient chromatographic separation without the complications arising from solid supports. The method is illustrated by the microanalytical separation of dinitrophenyl amino acids and can be used on a preparative scale.
我们开发了一种新的逆流色谱法，该方法在离心场中采用垂直螺旋管。螺旋管的布置方式使其在公转时不旋转，从而无需旋转密封。当旋转管中充满任一相，并将另一相沿正确方向引入管中时，会形成一种平衡状态，其中两相在盘管内被分成多个交替的段。随着流动相通过管的另一端稳定洗脱出来，每一相随着旋转来回振荡。因此，引入管中的溶质会经历快速分配过程，从而实现高效的色谱分离，而不会出现固体支持物带来的复杂问题。该方法通过二硝基苯基氨基酸的微量分析分离得到了说明，并且可用于制备规模。
