Ito Y, Bowman R L
J Chromatogr. 1977 Jun 11;136(2):189-98. doi: 10.1016/s0021-9673(00)86271-8.
The capability of a simple countercurrent chromatographic scheme for obtaining high-resolution preparative-scale separations was demonstrated with the separations of a series of dinitrophenyl (DNP) amino acids and peptides. Basic studies on stationary phase retention and partition efficiency with a low-viscosity chloroform-acetic acid-0.1 N hydrochloric acid (2:2:1) phase system together with the results previously obtained with a vascous n-butanol system suggest a general applicability of low-interfacial tension phase systems by the present method.
通过一系列二硝基苯基（DNP）氨基酸和肽的分离，证明了一种简单的逆流色谱方案用于获得高分辨率制备规模分离的能力。使用低粘度氯仿 - 乙酸 - 0.1N盐酸（2:2:1）相系统对固定相保留和分配效率进行的基础研究，以及先前在粘性正丁醇系统中获得的结果表明，本方法中低界面张力相系统具有普遍适用性。
