Henningsen K W, Boynton J E
J Cell Biol. 1970 Feb;44(2):290-304. doi: 10.1083/jcb.44.2.290.
Sequential changes occurring in the etioplasts of the primary leaf of 7-day-old dark-grown barley seedlings upon continuous illumination with 20 lux have been investigated by electron microscopy, in vivo spectrophotometry, and thin-layer chromatography. Following photoconversion of the protochlorophyllide pigment to chlorophyllide and the structural transformation of the crystalline prolamellar bodies, the tubules of the prolamellar bodies are dispersed into the primary lamellar layers. As both chlorophyll a and b accumulate, extensive formation of grana takes place. After 4 hr of greening, protochlorophyllide starts to reaccumulate, and concomitantly both large and small crystalline prolamellar bodies are formed. This protochlorophyllide is rapidly photoconverted upon exposure of the leaves to high light intensity, which also effects a rapid reorganization of the recrystallized prolamellar bodies into primary lamellar layers.
利用电子显微镜、活体分光光度法和薄层色谱法，研究了7日龄黑暗生长的大麦幼苗第一片叶子的黄化质体在持续20勒克斯光照下发生的一系列变化。原叶绿素酸酯色素光转化为叶绿素酸酯以及晶体原片层体发生结构转变后，原片层体的小管分散到初级片层中。随着叶绿素a和叶绿素b的积累，大量基粒开始形成。绿化4小时后，原叶绿素酸酯开始重新积累，同时形成了大的和小的晶体原片层体。当叶片暴露于高光强度下时，这种原叶绿素酸酯会迅速发生光转化，这也会使重新结晶的原片层体迅速重新组织成初级片层。
