关于伞藻质体间DNA数量存在差异的证据。
Evidence for variation in the quantity of DNA among plastids of Acetabularia.
作者信息
Woodcock C L, Bogorad L
出版信息
J Cell Biol. 1970 Feb;44(2):361-75. doi: 10.1083/jcb.44.2.361.
Abstract
The DNA content of individual plastids of the giant unicellular algae Acetabularia mediterranea, and Polyphysa cliftoni was studied. Four methods were used for localizing DNA: acridine orange staining, radioautography following actinomycin D-(3)H treatment, electron microscopy of thin tissue sections, and electron microscopy of osomotically disrupted plastids. With each method, DNA was readily detected in 20-35% of plastids, but no DNA was observed in the remaining 65-80%. The results further showed that in those plastids with detectable DNA the amount of DNA present was variable. The sensitivity and reliability of the localization methods are discussed, and the possible implications of these findings are considered.
摘要
对巨型单细胞藻类地中海伞藻和克利夫顿多藻单个质体的DNA含量进行了研究。使用了四种方法来定位DNA：吖啶橙染色、放线菌素D-（3）H处理后的放射自显影、薄组织切片的电子显微镜检查以及渗透破碎质体的电子显微镜检查。使用每种方法，在20%-35%的质体中很容易检测到DNA，但在其余65%-80%的质体中未观察到DNA。结果进一步表明，在那些可检测到DNA的质体中，存在的DNA量是可变的。讨论了定位方法的灵敏度和可靠性，并考虑了这些发现可能的影响。
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