Maruyama Y, Kusaka M, Mori J, Horikawa A, Hasegawa Y
J Chromatogr. 1979 Oct 11;164(2):121-7. doi: 10.1016/s0378-4347(00)81181-0.
An improved purification procedure is described for the simultaneous assay of endogenous choline and acetylcholine by pyrolysis gas chromatography, particularly for providing a simple and effective method for propionylation of choline in the presence of acetylcholine. The reaction was carried out in acetonitrile solution prepared by dissolving the evaporated residue of the supernatant of brain homogenate. Thus samples for propionylation were prepared without the use of ion-exchange chromatography.
描述了一种改进的纯化程序，用于通过热解气相色谱法同时测定内源性胆碱和乙酰胆碱，特别是为在乙酰胆碱存在下胆碱的丙酰化提供一种简单有效的方法。反应在通过溶解脑匀浆上清液的蒸发残余物制备的乙腈溶液中进行。因此，无需使用离子交换色谱法即可制备用于丙酰化的样品。
