Stavinoha W B, Weintraub S T
Science. 1974 Mar 8;183(4128):964-5. doi: 10.1126/science.183.4128.964.
The free choline concentration in the rat brain was found to be 26.3 nanomoles per gram of brain tissue. This value was obtained through use of 6-second microwave irradiation for killing animals and inactivating enzymes, followed by a pyrolysis-gas chromatographic assay procedure. The identities of compounds measured from brain samples were verified by mass spectrometry.
大鼠脑中游离胆碱的浓度为每克脑组织26.3纳摩尔。该值是通过使用6秒微波辐射杀死动物并使酶失活,然后采用热解气相色谱分析程序获得的。从脑样本中测得的化合物的身份通过质谱法进行了验证。