Almond J W, Haymerle H A, Felsenreich V D, Reeve P
J Gen Virol. 1979 Dec;45(3):611-21. doi: 10.1099/0022-1317-45-3-611.
Structural and virus-induced infected cell polypeptides of several strains of influenza B virus were examined by high resolution polyacrylamide gel electrophoresis and shown to be directly analogous to those of the influenza A viruses. Eight structural polypeptides, P1, P2, P3, HA1, HA2, NA, NP and M were observed in purified virus and at least two additional polypeptides, HA and NS could be detected in infected MDCK cells. The three P proteins plus NP were shown to be associated with RNA-dependent RNA polymerase activity and HA, HA1, HA2 and NA were shown to be glycosylated. Like the influenza A viruses, migrational differences of some of the infected cell polypeptides could be observed between different B strains. Investigation of a time course of virus replication failed to show any temporal control of protein synthesis in the infected cell.
通过高分辨率聚丙烯酰胺凝胶电泳对几株乙型流感病毒的结构及病毒诱导的感染细胞多肽进行了检测，结果表明它们与甲型流感病毒的多肽直接类似。在纯化病毒中观察到了8种结构多肽，即P1、P2、P3、HA1、HA2、NA、NP和M，并且在感染的MDCK细胞中可检测到至少另外两种多肽，即HA和NS。已证明三种P蛋白加NP与依赖RNA的RNA聚合酶活性相关，且HA、HA1、HA2和NA被证明是糖基化的。与甲型流感病毒一样，在不同的乙型流感病毒株之间可观察到一些感染细胞多肽的迁移差异。对病毒复制时间进程的研究未能显示感染细胞中蛋白质合成有任何时间控制。
