Elliott R M, Yuanji G, Desselberger U
J Gen Virol. 1984 Nov;65 ( Pt 11):1873-80. doi: 10.1099/0022-1317-65-11-1873.
MDCK cells were infected with six human influenza C virus strains (isolated between 1947 and 1981) and seven pig influenza C virus strains (isolated in 1981 and 1982) and the virus-specific polypeptides were compared by SDS-polyacrylamide gel electrophoresis and one-dimensional peptide mapping. The major structural polypeptides, i.e. glycoprotein (gp88), nucleoprotein (NP), and membrane protein (M), and one non-structural polypeptide were identified in all strains by radiolabelling infected cells with [35S]methionine. No differences in the electrophoretic migration of the M proteins or NS proteins were observed. The two earliest human isolates, C/Taylor/1233/47 and C/Great Lakes/1167/54, had faster migrating NP proteins, and another human strain, C/Georgia/1/69, displayed a faster migrating gp88. Minor differences in the one-dimensional peptide maps produced by partial digestion of the M proteins with V8 protease were observed between the human and pig isolates, while more marked differences were noted in the peptide maps of the glycoproteins of the C/Georgia/1/69, C/Yamagata/10/81 and C/Yamagata/11/81 viruses compared to the other human strains and the pig strains. The overall conclusion is that the proteins of human influenza C viruses isolated over a 35 year period and those of recent pig influenza C virus isolates are highly conserved.
用6株人C型流感病毒株（1947年至1981年间分离）和7株猪C型流感病毒株（1981年和1982年分离）感染MDCK细胞，并通过SDS-聚丙烯酰胺凝胶电泳和一维肽图分析比较病毒特异性多肽。通过用[35S]甲硫氨酸对感染细胞进行放射性标记，在所有毒株中鉴定出主要结构多肽，即糖蛋白（gp88）、核蛋白（NP）和膜蛋白（M），以及一种非结构多肽。未观察到M蛋白或NS蛋白电泳迁移的差异。最早的两株人分离株C/Taylor/1233/47和C/Great Lakes/1167/54的NP蛋白迁移速度更快，另一株人毒株C/Georgia/1/69的gp88迁移速度更快。用人和猪分离株对M蛋白用V8蛋白酶进行部分消化所产生的一维肽图存在微小差异，而与其他人类毒株和猪毒株相比，C/Georgia/1/69、C/Yamagata/10/81和C/Yamagata/11/81病毒糖蛋白的肽图差异更为明显。总体结论是，在35年期间分离的人C型流感病毒的蛋白质与近期猪C型流感病毒分离株的蛋白质高度保守。
