Hugentobler A L, Schild G C, Oxford J S
Arch Virol. 1981;69(3-4):197-207. doi: 10.1007/BF01317335.
The electrophoretic migration rates of structural and non-structural polypeptides of 38 influenza B viruses isolated in epidemics in 1978-1980 and antigenically closely related to B/Singapore/222/79 virus were compared using high resolution SDS polyacrylamide gels. Thirty of the viruses could be distinguished from the prototype B/Singapore/222/79 virus by electrophoretic migration rate differences in HA, 17 by differences in NP and 27 by differences in mobility of the NS 1 polypeptide. Mobility differences of NP, NS 1 and HA polypeptides was noted in influenza B viruses isolated in the UK in the same year. In addition, electrophoretic mobility of 32P labeled virus RNAs varied for certain UK isolates and indicated heterogeneity in genes 2, 3, 4 and 8 coding for polymerase proteins 2 and 1, nucleoprotein (NP) and non-structural protein (NS 1) respectively.
使用高分辨率SDS聚丙烯酰胺凝胶，比较了1978 - 1980年流行期间分离出的、在抗原性上与B/新加坡/222/79病毒密切相关的38株乙型流感病毒的结构和非结构多肽的电泳迁移率。其中30株病毒可通过血凝素（HA）电泳迁移率差异与原型B/新加坡/222/79病毒区分开来，17株通过核蛋白（NP）差异区分，27株通过非结构蛋白1（NS 1）多肽迁移率差异区分。同年在英国分离出的乙型流感病毒中也发现了NP、NS 1和HA多肽的迁移率差异。此外，某些英国分离株的32P标记病毒RNA的电泳迁移率有所不同，这表明分别编码聚合酶蛋白2和1、核蛋白（NP）和非结构蛋白（NS 1）的基因2、3、4和8存在异质性。
