Nayak N C, Mital I, Dhar A, Chopra P, Das P K
Br J Exp Pathol. 1975 Apr;56(2):113-8.
The effects of variable degrees of liver regeneration induced by two-thirds partial hepatectomy or intoxication with different doses of carbon tetrachloride (CCl4) on increased production of alpha foetoprotein (AFP) have been studied in very young (5-6 weeks) and older (15 weeks and more) rats by counter-immunoelectrophoresis (sensitivity down to 250 ng/ml). In the young animal adequate regeneration following two-thirds hepatectomy as well as 100 myl CCl4 successfully induced large increases in serum AFP levels. Smaller doses as well as a large fatal dose of the toxin, all of which resulted in inadequate regenerative activity, failed to excite AFP synthesis and secretion up to detectable levels. The adult animal did not show detectable AFP with any of these procedures. However, necrosis induced in the regenerating adult liver 24 h following partial hepatectomy did result in the detection of small amounts of protein in the serum. It is concluded that in hepatic regeneration of this species, the age of the animal as well as the magnitude of regenerating activity in the liver are critical in the occurrence of high levels of serum AFP.
通过对流免疫电泳法（灵敏度低至250纳克/毫升），研究了三分之二部分肝切除术或不同剂量四氯化碳（CCl4）中毒诱导的不同程度肝脏再生对幼龄（5 - 6周）和老龄（15周及以上）大鼠甲胎蛋白（AFP）产量增加的影响。在幼龄动物中，三分之二肝切除术后以及100微升CCl4处理后充分的肝脏再生成功诱导血清AFP水平大幅升高。较小剂量以及大剂量致死毒素，均导致再生活性不足，未能激发AFP合成和分泌至可检测水平。成年动物采用上述任何方法均未检测到可检测水平的AFP。然而，部分肝切除术后24小时在成年再生肝脏中诱导的坏死确实导致血清中检测到少量蛋白质。结论是，在该物种的肝脏再生中，动物年龄以及肝脏再生活动的程度对于血清AFP高水平的出现至关重要。
