Manney T R
J Bacteriol. 1970 May;102(2):483-8. doi: 10.1128/jb.102.2.483-488.1970.
Indole-accumulating and indole-utilizing tryptophan synthetase mutants of Saccharomyces cerevisiae were found to form complementing diploids with unusual growth properties. These strains grew at nearly the wild-type exponential rate, but only after an abnormally prolonged lag period of a duration that depended on the initial cell density. The exaggerated lag was reduced by adding low concentrations of indole in the medium. The growth properties were interpreted to be a consequence of the inability of these strains to synthesize tryptophan by the normal mechanism.
酿酒酵母中积累吲哚和利用吲哚的色氨酸合成酶突变体被发现能形成具有异常生长特性的互补二倍体。这些菌株以接近野生型的指数速率生长，但只有在异常延长的延迟期之后才开始生长，延迟期的持续时间取决于初始细胞密度。通过在培养基中添加低浓度的吲哚可以减少这种过度的延迟。这些生长特性被解释为是由于这些菌株无法通过正常机制合成色氨酸的结果。
