酿酒酵母色氨酸合成酶突变体中等位基因互补的两种机制。
Two mechanisms of allelic complementation among tryptophan synthetase mutants of Saccharomyces cerevisiae.
作者信息
Duntze W, Manney T R
出版信息
J Bacteriol. 1968 Dec;96(6):2085-93. doi: 10.1128/jb.96.6.2085-2093.1968.
Abstract
Two different types of allelic complementation were observed in tryptophan synthetase mutants of the yeast Saccharomyces cerevisiae. Each type is associated with a different mechanism for the enzymatic conversion of indole-3-glycerol phosphate (InGP) to tryptophan. Mechanism I is utilized by a hybrid tryptophan synthetase that resembles, but is not identical with, the wild-type enzyme. Mechanism II is due to a sequential conversion of InGP to free indole, and indole to tryptophan. Two partially active mutant enzymes rather than a single hybrid enzyme catalyze the sequential reaction steps. This is an example of intracellular cross-feeding. The quantitative evaluation of mechanism II leads to the conclusion that tryptophan synthetase in yeast is most likely a dimer of two identical subunits.
摘要
在酿酒酵母色氨酸合成酶突变体中观察到两种不同类型的等位基因互补。每种类型都与吲哚 - 3 - 甘油磷酸（InGP）酶促转化为色氨酸的不同机制相关。机制I由一种杂合色氨酸合成酶利用，该酶与野生型酶相似但不相同。机制II是由于InGP顺序转化为游离吲哚，然后吲哚再转化为色氨酸。两个部分活性的突变酶而非单个杂合酶催化这一系列反应步骤。这是细胞内交叉喂养的一个例子。对机制II的定量评估得出结论，酵母中的色氨酸合成酶很可能是由两个相同亚基组成的二聚体。
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本文引用的文献
1
ON THE SEPARATION OF THE TRYPTOPHAN SYNTHETASE OF ESCHERICHIA COLI INTO TWO PROTEIN COMPONENTS.关于大肠杆菌色氨酸合成酶分离为两种蛋白质组分的研究
Proc Natl Acad Sci U S A. 1958 Dec 15;44(12):1161-70. doi: 10.1073/pnas.44.12.1161.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
ACTION OF A SUPER-SUPPRESSOR IN YEAST IN RELATION TO ALLELIC MAPPING AND COMPLEMENTATION.酵母中一种超级抑制因子与等位基因定位及互补作用的关系
Genetics. 1964 Jul;50(1):109-21. doi: 10.1093/genetics/50.1.109.
4
ALLELIC MAPPING IN YEAST BY X-RAY-INDUCED MITOTIC REVERSION.通过X射线诱导的有丝分裂逆转在酵母中进行等位基因定位
Science. 1964 Feb 7;143(3606):581-3. doi: 10.1126/science.143.3606.581.
5
Studies on the mechanism of the tryptophan synthetase reaction.色氨酸合成酶反应机制的研究。
Biochim Biophys Acta. 1962 Aug 13;62:279-93. doi: 10.1016/0006-3002(62)90041-0.
6
The fine structure of the loci tryC and tryD of Salmonella typhimurium. I. Determination of the order of mutational sites by three-point transduction tests.鼠伤寒沙门氏菌色氨酸操纵子tryC和tryD位点的精细结构。I. 通过三点转导试验确定突变位点的顺序。
Genetics. 1962 Apr;47(4):469-82. doi: 10.1093/genetics/47.4.469.
7
The tryptophan synthetase system.色氨酸合成酶系统。
Bacteriol Rev. 1960 Jun;24(2):221-45. doi: 10.1128/br.24.2.221-245.1960.
8
Complementation between alleles of the Td locus in Neurospora crassa.粗糙脉孢菌中Td基因座等位基因间的互补作用。
Proc Natl Acad Sci U S A. 1961 Jan 15;47(1):72-7. doi: 10.1073/pnas.47.1.72.
9
The exclusion of free indole as an intermediate in the biosynthesis of tryptophan in Neurospora crassa.粗糙脉孢菌中游离吲哚作为色氨酸生物合成中间体的排除。
Biochim Biophys Acta. 1958 Jun;28(3):640-1. doi: 10.1016/0006-3002(58)90533-x.
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