Hoffman D J, Moore J M
Teratology. 1979 Dec;20(3):453-61. doi: 10.1002/tera.1420200315.
The embryotoxic potential of external applications of methyl mercury on mallard eggs was investigated to assess the possible impact of mercury transferred from the plumage of effluent-contaminated aquatic birds to their eggs. Eggs were treated on day 3 of development with microliter applications of methyl mercury that was dissolved with ethyl acetate into an aliphatic hydrocarbon vehicle. Mercury analysis by atomic absorption indicated that almost half of the mercury applied entered the eggs past the shell membranes within several days of treatment. Most mortality occurred within this period at doses of 9 microgram of mercury per egg or higher. Decreased embryonic growth resulted with similar doses. A significant incidence of malformations occurred at a dose of 1 microgram per egg. These malformations were mainly minor skeletal aberrations and incomplete ossification. With higher doses of mercury, defects included gross external ones such as micromella, gastroschisis, and eye and brain defects. Application of the aliphatic hydrocarbon vehicle did not result in any of these defects.
研究了甲基汞外用对绿头鸭蛋的胚胎毒性潜力，以评估受污水污染的水鸟羽毛中的汞转移到其卵中可能产生的影响。在发育第3天，用微升量的甲基汞处理卵，甲基汞用乙酸乙酯溶解在脂肪烃载体中。原子吸收法进行的汞分析表明，处理后几天内，施用的汞几乎有一半通过壳膜进入了卵中。在此期间，大多数死亡发生在每枚卵含9微克或更高剂量汞的情况下。相似剂量导致胚胎生长减缓。每枚卵含1微克汞的剂量时出现了显著的畸形发生率。这些畸形主要是轻微的骨骼畸变和不完全骨化。汞剂量更高时，缺陷包括明显的外部缺陷，如微小畸形、腹裂以及眼睛和脑部缺陷。施用脂肪烃载体未导致任何这些缺陷。
