通过薄层色谱法和荧光分析法测定生物体液中的单个胆汁酸。
Determination of individual bile acids in biological fluids by thin-layer chromatography and fluorimetry.
作者信息
Panveliwalla D, Lewis B, Wootton I D, Tabaqchali S
出版信息
J Clin Pathol. 1970 May;23(4):309-14. doi: 10.1136/jcp.23.4.309.
Abstract
A method is described for the separation and determination of individual bile acids in serum and in intestinal contents, employing thin-layer chromatography and fluorimetry. The mean recovery from intestinal juice was 94% and from serum 82%. Normal values for the individual bile acids of serum and examples of clinical applications of the method are given.
摘要
本文描述了一种采用薄层色谱法和荧光分析法分离并测定血清和肠内容物中各胆汁酸的方法。肠液的平均回收率为94%，血清的平均回收率为82%。文中给出了血清中各胆汁酸的正常值以及该方法的临床应用实例。
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