Mohrig W, Schittek D
Acta Biol Med Ger. 1979;38(7):953-8.
The ability of insect blood cells to ingest all kinds of synthetic particles and also a wide range of microorganisms in a very short time after injection has up to now been regarded as a phagocytic function without any humoral mediators. In a phagocytosis model with latex beads and nonhagocytosable cells of Bacillus thuringiensis subtoxicus, we are able to demonstrate the existence of lymphokine-like factors, which intervene in cellular defence reactions of insect. The following results were obtained: 1. Immediately after injection of latex beads, normally non-phagocytosable cells of Bacillus thuringiensis subtoxicus are phagocytosed. 2. Cell-free haemolymph of larvae of Galleria mellonella previously injected with latex beads, stimulates in new larvae phagocytosis of Bacillus thuringiensis subtoxicus after transfusion. 3. The fractionation of homogenates of latex-treated larvae on Sephadex G 50 shows two fractions which stimulate phagocytosis. We suppose that the appearance of these phagocytosis-stimulating factors is the result of a successful recognition of foreign material.
昆虫血细胞在注射后很短时间内摄取各种合成颗粒以及多种微生物的能力，至今一直被视为一种没有任何体液介质参与的吞噬功能。在一个使用乳胶珠和苏云金芽孢杆菌无毒亚种不可吞噬细胞的吞噬作用模型中，我们能够证明存在类淋巴因子，它们会干预昆虫的细胞防御反应。得到了以下结果：1. 注射乳胶珠后，苏云金芽孢杆菌无毒亚种通常不可吞噬的细胞会立即被吞噬。2. 先前注射过乳胶珠的大蜡螟幼虫的无细胞血淋巴，在输血后会刺激新幼虫对苏云金芽孢杆菌无毒亚种的吞噬作用。3. 用葡聚糖凝胶G 50对经乳胶处理的幼虫匀浆进行分级分离，显示出两个刺激吞噬作用的组分。我们推测这些吞噬作用刺激因子的出现是成功识别异物的结果。
