由β-内酰胺抗生素形成的聚合物的抗原特性。

Antigenic properties of polymers formed by beta-lactam antibiotics.

作者信息

Munro A C, Dewdney J M, Smith H, Wheeler A W

出版信息

Int Arch Allergy Appl Immunol. 1976;50(2):192-205. doi: 10.1159/000231496.

Abstract

Purified polymers have been isolated from 6-aminopenicillanic acid and from semi-synthetic penicillins and cephalosporins. All the polymers were shown to react with rabbit antibody of penicillins and cephalosporins. All the polymers were shown to react with rabbit antibody of penicilloyl specificity, as demonstrated by passive cutaneous anaphylaxis in guinea pigs, and the reactions were shown to be penicilloyl-specific by hapten inhibition experiments. The cephalosporin-derived polymers in addition reacted with rabbit antibodies raised to the corresponding cephalosporin conjugates of bovine gamma-globulin. Using direct skin tests, passive cutaneous anaphylaxis and an in vitro assay, no evidence was obtained that the polymers induced the formation of specific antibodies in baboons, guinea pigs and rabbits, but in baboons the induction of cell-mediated immunity, demonstrable by delayed skin test reactions, was shown.

摘要

已从6-氨基青霉烷酸以及半合成青霉素和头孢菌素中分离出纯化的聚合物。所有聚合物均显示能与兔抗青霉素和头孢菌素抗体发生反应。所有聚合物均显示能与具有青霉噻唑特异性的兔抗体发生反应，这在豚鼠的被动皮肤过敏反应中得到证实，并且通过半抗原抑制实验表明这些反应具有青霉噻唑特异性。此外，头孢菌素衍生的聚合物还能与针对牛γ-球蛋白相应头孢菌素缀合物产生的兔抗体发生反应。通过直接皮肤试验、被动皮肤过敏反应和体外试验，未获得聚合物在狒狒、豚鼠和兔子体内诱导形成特异性抗体的证据，但在狒狒中显示出可通过迟发性皮肤试验反应证明的细胞介导免疫的诱导。