Smith H, Dewdney J M, Wheeler A W
Immunology. 1971 Sep;21(3):527-33.
Concentrated aqueous solutions of some β-lactam antibiotics were stored for 14 days. Samples were then subjected to gel filtration and fractions in the high molecular weight regions of the eluate were collected and lyophilized. The yields of materials from solutions of various β-lactam antibiotics were compared and the antigenicity of the materials was assessed by their ability to react with antibodies of benzylpenicilloyl specificity raised in rabbits and tested in guinea-pigs by passive cutaneous anaphylaxis.
将一些β-内酰胺抗生素的浓水溶液储存14天。然后对样品进行凝胶过滤，收集洗脱液中高分子量区域的级分并冻干。比较了各种β-内酰胺抗生素溶液中物质的产率，并通过它们与在兔体内产生的苄青霉素特异性抗体反应的能力来评估这些物质的抗原性，该抗体通过豚鼠被动皮肤过敏反应进行测试。
