Hard G C
Br J Exp Pathol. 1970 Feb;51(1):97-105.
Some of the biochemical properties of mouse peritoneal macrophages immune to were characterised. Total cellular protein of immune cells exceeded that of normal phagocytes by 1·85 times. The activities of 7 hydrolytic enzymes, acid phosphatase, β-glucuronidase, Cathepsin D, lysozyme, BPN-ase, MN esterase and aryl sulphatase were measured in lysed cell suspensions and monolayer cultures. Immune macrophages possessed substantially higher levels of these enzymes than did normal cells. No one enzyme was significantly more associated with the development of cellular immunity than another. Resting immune macrophages consumed significantly less oxygen than normal cells required but were twice as active in glycolysis. ATP levels, in agreement, were 5 times higher in normal macrophages whereas ATP-ase activities were equivalent. Normal macrophages were approximately twice as active in protein synthesis measured by the incorporation of C L-glycine by monolayer cultures than were immune cells. These results were considered in the light of known morphological differences between the 2 cells noted at the ultrastructural level.
对免疫后的小鼠腹腔巨噬细胞的一些生化特性进行了表征。免疫细胞的总细胞蛋白比正常吞噬细胞高出1.85倍。在裂解的细胞悬液和单层培养物中测量了7种水解酶的活性，即酸性磷酸酶、β-葡萄糖醛酸酶、组织蛋白酶D、溶菌酶、BPN酶、MN酯酶和芳基硫酸酯酶。免疫巨噬细胞中这些酶的水平明显高于正常细胞。没有一种酶比另一种酶与细胞免疫的发展更显著相关。静止的免疫巨噬细胞消耗的氧气明显少于正常细胞所需，但糖酵解活性是正常细胞的两倍。同样，正常巨噬细胞中的ATP水平高出5倍，而ATP酶活性相当。通过单层培养物中C L-甘氨酸的掺入来测量，正常巨噬细胞在蛋白质合成方面的活性大约是免疫细胞的两倍。根据在超微结构水平上观察到的这两种细胞之间已知的形态学差异对这些结果进行了考量。
