来自反应性和无反应性小鼠的受刺激和未受刺激巨噬细胞对多肽抗原的摄取和降解
Uptake and degradation of a polypeptide antigen by stimulated and unstimulated macrophages from responder and non-responder mice.
作者信息
Gallily R, Eliahu H
出版信息
Immunology. 1974 Mar;26(3):603-12.
An increase in the level of acid phosphatase, β-glucuronidase and cathepsin D in macrophages was obtained by cultivating the cells in medium containing high serum concentration and by injecting macrophage donors with thioglycollate. A high level of the three hydrolytic enzymes in the stimulated macrophages does not correlate with a high uptake or degradation of the polypeptide antigen (TG)-PRO--L. Moreover, macrophages with a low level of enzymes (unstimulated cells) pinocytose and degrade (TG)-PRO--L more efficiently than macrophages with a high content of hydrolases. Unstimulated macrophages from responder (SJL) mice pinocytose and degrade (TG)-PRO--L more efficiently than macrophages from the non-responder (DBA/1) animals although the two cell populations had similar levels of hydrolases.
通过在含有高血清浓度的培养基中培养细胞以及给巨噬细胞供体注射巯基乙酸盐，可使巨噬细胞中的酸性磷酸酶、β-葡萄糖醛酸酶和组织蛋白酶D水平升高。受刺激巨噬细胞中这三种水解酶的高水平与多肽抗原（TG）-PRO-L的高摄取或降解并无关联。此外，酶水平低的巨噬细胞（未受刺激的细胞）比水解酶含量高的巨噬细胞更有效地胞饮和降解（TG）-PRO-L。来自反应者（SJL）小鼠的未受刺激巨噬细胞比来自无反应者（DBA/1）动物的巨噬细胞更有效地胞饮和降解（TG）-PRO-L，尽管这两种细胞群体的水解酶水平相似。
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