Otto K, Höffken H, Soost H J
J Histochem Cytochem. 1979 Jan;27(1):14-8. doi: 10.1177/27.1.86560.
A preparation procedure, aiming at monolayer deposition of cervical exfoliative material on glass slides for high resolution prescreening has been developed. The main features of this procedure are centrifugal deposition after suspension and sedimentation of samples over isopycnic medium of 1.026 density. Fractioning of the separation column after centrifugation at 50 X g yields two preparations with leukocytes, bacteria and cellular debris predominantly located on the first slide and epithelial cells on the second one. The degree of spatial cellular isolation as well as the amount of diagnostically relevant cells per slide seem to fit the requirements of automated high resolution analysis.
已经开发出一种制备程序，旨在将宫颈脱落物质单层沉积在载玻片上以进行高分辨率预筛查。该程序的主要特点是在样品悬浮并沉淀在密度为1.026的等密度介质上之后进行离心沉积。以50×g离心后，分离柱的分级产生两种制剂，白细胞、细菌和细胞碎片主要位于第一张载玻片上，上皮细胞位于第二张载玻片上。每张载玻片上细胞的空间隔离程度以及诊断相关细胞的数量似乎符合自动高分辨率分析的要求。
