在感染梅森-辉瑞病毒的培养物中检测猿猴C型颗粒。

Detection of simian type-C particles in Mason-Pfizer virus infected cultures.

作者信息

Ahmed M, Schidlovsky G, Larson D L, Manousos M, Mayyasi S A

出版信息

J Gen Virol. 1976 Jan;30(1):11-20. doi: 10.1099/0022-1317-30-1-11.

Abstract

A simian type-C virus has been detected in cultures chronically infected with Mason-Pfizer monkey virus (M-PMV). Simultaneous budding of M-PMV and type-C virus particles from the same cells was observed in cultures incubated at 37 or 40 degrees C. However, the frequency of such cells was greater in cultures grown at 40 degrees C. Although clusters of type-C viral buds were seen at the surface of the cells, extracellular mature type-C particles in cell pellets or concentrated virus preparations were very rarely found. The increase in frequency of type-C buds was found to be transitory since cultures adapted to growing at the high temperature demonstrated budding type-C particles only occasionally. Cultures producing type-C buds were found to contain, in addition to M-PMV antigens, serological activity with polyvalent antisera produced against multiple structural components of endogenous baboon virus (BV) or simian sarcoma virus (SiSV). The reactivity, however, was found not to be serologically related to the major SiSV P28 core protein.

摘要

在长期感染梅森- Pfizer猴病毒（M-PMV）的培养物中检测到一种猿猴C型病毒。在37或40摄氏度培养的培养物中，观察到M-PMV和C型病毒颗粒从同一细胞同时出芽。然而，在40摄氏度培养的培养物中，此类细胞的频率更高。虽然在细胞表面可见C型病毒芽簇，但在细胞沉淀或浓缩病毒制剂中很少发现细胞外成熟的C型颗粒。发现C型芽频率的增加是短暂的，因为适应在高温下生长的培养物仅偶尔显示出芽的C型颗粒。发现产生C型芽的培养物除了含有M-PMV抗原外，还与针对内源性狒狒病毒（BV）或猿猴肉瘤病毒（SiSV）的多种结构成分产生的多价抗血清具有血清学活性。然而，发现这种反应性与主要的SiSV P28核心蛋白在血清学上无关。