Ahmed M, Schidlovsky G, Harewood K R, Manousos M, Mayyasi S A
J Natl Cancer Inst. 1977 May;58(5):1515-8. doi: 10.1093/jnci/58.5.1515.
Production of infectious Mason-Pfizer monkey virus (M-PMV) was enhanced after treatment of the CMMT cell line with 2.5 x 10(-5) M dexamethasone phosphate (DXM). The reverse transcriptase (RT) activity and infectivity titers of treated culture fluids were enhanced by five- and tenfold, respectively. Along with stimulation of M-PMV synthesis, a simian type C virus (SCV) was also detected by electron microscopic and RT analyses. The SCV was serologically related to the endogenous baboon type C virus. 5-iododeoxyuridine (IUDR) also activated the SCV in the CMMT cell line while significantly inhibiting the production of infectious M-PMV. The activation of endogenous SCV by IUDR or DXM was transitory, since removal of these compounds from the growth medium resulted in the disappearance of SCV buds and the related RT activity; however, low levels of specific viral structural proteins continued to be synthesized intracellularly. Similarly, the enhancement of M-PMV production seen with DXM was lost when the treated cells were subcultured for 2 weeks in the absence of the hormone.
用2.5×10⁻⁵ M磷酸地塞米松（DXM）处理CMMT细胞系后，传染性马森 - 辉瑞猴病毒（M - PMV）的产量增加。处理后的培养液中逆转录酶（RT）活性和感染性滴度分别提高了五倍和十倍。在刺激M - PMV合成的同时，通过电子显微镜和RT分析还检测到一种猿猴C型病毒（SCV）。该SCV与内源性狒狒C型病毒存在血清学关系。5 - 碘脱氧尿苷（IUDR）也能激活CMMT细胞系中的SCV，同时显著抑制传染性M - PMV的产生。IUDR或DXM对内源性SCV的激活是短暂的，因为从生长培养基中去除这些化合物会导致SCV芽体和相关RT活性消失；然而，细胞内仍持续合成低水平的特定病毒结构蛋白。同样，当处理后的细胞在无激素条件下传代培养2周时，DXM所导致的M - PMV产量增加就会消失。
