Vorherr H, Munsick R A
J Clin Invest. 1970 Apr;49(4):828-36. doi: 10.1172/JCI106296.
Identifying posterior pituitary hormones in body fluids or neurohypophysial extracts was heretofore partially achieved by using pharmacologic potency ratios or semispecific inactivation by thioglycolate or enzymes. Production of antisera against oxytocin and lysine-vasopressin has prompted us to test their specificity against lysine-vasopressin, arginine-vasopressin, arginine-vasotocin, and oxytocin. In ethanol anesthetized rats, antidiuretic and milk-ejection activities were assayed for each peptide-antiserum combination after 0, 30, 60, and 90 min of incubation. Results indicate that (a) oxytocin antiserum inactivates oxytocin, but not arginine-vasopressin, lysine-vasopressin, or arginine-vasotocin; vasopressin antiserum inactivates arginine-vasopressin and lysine-vasopressin, but neither oxytocin nor arginine-vasotocin; (b) an identifiable antigenic site exists for each hormone; (c) relatively specific identifications of natural neurohypophysial peptides are possible using antisera and bioassays; (d) this method is promising for identifying neurohypophysial peptides in body fluids and pituitary extracts; and (e) active and passive immunization against oxytocin and vasopressin may increase our understanding of their physiologic functions.
此前，通过使用药理效价比或巯基乙酸盐或酶的半特异性灭活方法，可部分实现对体液或神经垂体提取物中垂体后叶激素的鉴定。抗催产素和赖氨酸加压素抗血清的产生促使我们测试它们对赖氨酸加压素、精氨酸加压素、精氨酸催产素和催产素的特异性。在乙醇麻醉的大鼠中，在孵育0、30、60和90分钟后，对每种肽 - 抗血清组合测定抗利尿和排乳活性。结果表明：（a）催产素抗血清可使催产素失活，但不能使精氨酸加压素、赖氨酸加压素或精氨酸催产素失活；加压素抗血清可使精氨酸加压素和赖氨酸加压素失活，但不能使催产素或精氨酸催产素失活；（b）每种激素都存在一个可识别的抗原位点；（c）使用抗血清和生物测定法可以对天然神经垂体肽进行相对特异性的鉴定；（d）这种方法有望用于鉴定体液和垂体提取物中的神经垂体肽；（e）针对催产素和加压素的主动和被动免疫可能会增进我们对其生理功能的理解。
