Freidman-Mor Z, Chalon J, Katz J S, Gorstein F, Turndorf H, Orkin L R
J Trauma. 1976 Jan;16(1):58-62. doi: 10.1097/00005373-197601000-00007.
Smears were made from tracheobronchial washings of patients undergoing general endotracheal anesthesia for surgery. Morphologically abnormal histiocytes were noticed in specimens obtained from subjects during hemorrhagic shock. These cells were overloaded with Papanicolaou stain (a greenish orange compound) which compressed the nucleus against the cell membrane. Cytochemical staining methods were undertaken to discover the composition of this substance. In secretions suctioned from ten patients in shock, large numbers of histiocytes were found to have ingested inorganic iron detectable by the Prussian blue method. Only two patients from a matched control group had smears in which this phenomenon was discovered. The maximum proportion of histiocytes containing Prussian blue granules was 40% for patients in shock and only 2% in normal controls. Histologic studies conducted on rats submitted to hemorrhagic shock were carried out to investigate this phenomenon. Iron-laden histiocytes were found in the kidneys, spleen, and lungs of both shocked and control animals. However, substantially more histiocytes containing Prussian blue positive granules were discovered in the lungs of rats in shock than in controls. It may, therefore, be that iron is deposited in the lungs in low flow states.
涂片取自接受全身气管内麻醉进行手术患者的气管支气管灌洗物。在出血性休克患者的标本中发现了形态异常的组织细胞。这些细胞被巴氏染色剂（一种绿橙色化合物）过度染色，导致细胞核被挤向细胞膜。采用细胞化学染色方法来发现这种物质的成分。在从10名休克患者吸出的分泌物中，发现大量组织细胞摄取了普鲁士蓝法可检测到的无机铁。在匹配的对照组中，只有2名患者的涂片发现了这种现象。休克患者中含普鲁士蓝颗粒的组织细胞最大比例为40%，而正常对照组仅为2%。对遭受出血性休克的大鼠进行组织学研究以调查这一现象。在休克和对照动物的肾脏、脾脏和肺中均发现了含铁的组织细胞。然而，休克大鼠肺中发现的含普鲁士蓝阳性颗粒的组织细胞明显多于对照组。因此，可能是在低血流状态下铁沉积在肺部。
