• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
In vitro transcription of 70S RNA by the RNA-directed DNA polymerase of Rouse sarcoma virus: lack of influence of RNase H.劳氏肉瘤病毒的RNA指导DNA聚合酶对70S RNA的体外转录：核糖核酸酶H的影响缺失
J Virol. 1975 Jan;17(1):291-5. doi: 10.1128/JVI.17.1.291-295.1976.
2
Extensive in vitro transcription of rous sarcoma virus RNA by avian myeloblastosis virus DNA polymerase and concurrent activation of the associated RNase H.禽成髓细胞瘤病毒DNA聚合酶对劳氏肉瘤病毒RNA进行广泛的体外转录，并同时激活相关的核糖核酸酶H。
J Virol. 1977 Sep;23(3):659-68. doi: 10.1128/JVI.23.3.659-668.1977.
3
New procedure for the direct analysis of in vitro reverse transcription of Rous sarcoma virus RNA.劳氏肉瘤病毒RNA体外逆转录直接分析的新方法。
J Virol. 1977 Apr;22(1):118-29. doi: 10.1128/JVI.22.1.118-129.1977.
4
DNA polymerase of murine sarcoma-leukemia virus: lack of detectable RNase H and low activity with viral RNA and natural DNA templates.鼠肉瘤-白血病病毒的DNA聚合酶：未检测到核糖核酸酶H，对病毒RNA和天然DNA模板的活性较低。
J Virol. 1973 Dec;12(6):1512-21. doi: 10.1128/JVI.12.6.1512-1521.1973.
5
Properties of oncornavirus RNA-directed DNA polymerase, the RNA template, and the intracellular products formed early during infection and cell transformation.致癌RNA病毒RNA指导的DNA聚合酶、RNA模板以及感染和细胞转化早期形成的细胞内产物的特性。
Cold Spring Harb Symp Quant Biol. 1975;39 Pt 2:975-85. doi: 10.1101/sqb.1974.039.01.112.
6
Effect of viral RNase H on the avian sarcoma viral genome during early transcription in vitro.病毒核糖核酸酶H在体外早期转录过程中对禽肉瘤病毒基因组的影响。
J Virol. 1979 Sep;31(3):630-8. doi: 10.1128/JVI.31.3.630-638.1979.
7
In vitro transcription of DNA from the 70S RNA of Rous sarcoma virus: identification and characterization of various size classes of DNA transcripts.来自劳氏肉瘤病毒70S RNA的DNA体外转录：不同大小类别的DNA转录本的鉴定与表征
J Virol. 1975 Nov;16(5):1220-8. doi: 10.1128/JVI.16.5.1220-1228.1975.
8
Transcription of DNA from the 70S RNA of Rous sarcoma virus. I. Identification of a specific 4S RNA which serves as primer.劳斯肉瘤病毒70S RNA的DNA转录。I. 作为引物的一种特定4S RNA的鉴定。
J Virol. 1974 May;13(5):1126-33. doi: 10.1128/JVI.13.5.1126-1133.1974.
9
Initiation of DNA synthesis by the avian oncornavirus RNA-directed DNA polymerase: tryptophan tRNA as the major species of primer RNA.禽肿瘤病毒RNA指导的DNA聚合酶引发DNA合成：色氨酸tRNA作为主要的引物RNA种类。
J Virol. 1976 Mar;17(3):1049-51. doi: 10.1128/JVI.17.3.1049-1051.1976.
10
RNase H hydrolysis of the 5' terminus of the avian sarcoma virus genome during reverse transcription.禽肉瘤病毒基因组在逆转录过程中5'末端的核糖核酸酶H水解作用。
Nature. 1978 Mar 9;272(5649):181-4. doi: 10.1038/272181a0.

引用本文的文献

1
Kinetics of human immunodeficiency virus type 1 reverse transcription in blood mononuclear phagocytes are slowed by limitations of nucleotide precursors.1型人类免疫缺陷病毒在血液单核吞噬细胞中的逆转录动力学因核苷酸前体的限制而减慢。
J Virol. 1994 Feb;68(2):1258-63. doi: 10.1128/JVI.68.2.1258-1263.1994.
2
Endoribonuclease activity associated with animal RNA viruses.与动物RNA病毒相关的核糖核酸内切酶活性
J Virol. 1978 Jan;25(1):274-84. doi: 10.1128/JVI.25.1.274-284.1978.
3
In vitro synthesis of infectious transforming DNA by the avian sarcoma virus reverse transcriptase.禽肉瘤病毒逆转录酶体外合成感染性转化DNA
J Virol. 1979 Jan;29(1):242-9. doi: 10.1128/JVI.29.1.242-249.1979.
4
Reverse transcriptase of RNA tumor viruses. V. In vitro proteolysis of reverse transcriptase from avian myeloblastosis virus and isolation of a polypeptide manifesting only RNase H activity.RNA肿瘤病毒的逆转录酶。V. 禽成髓细胞瘤病毒逆转录酶的体外蛋白水解作用及仅表现核糖核酸酶H活性的一种多肽的分离
J Virol. 1978 Feb;25(2):652-63. doi: 10.1128/JVI.25.2.652-663.1978.
5
Extensive in vitro transcription of rous sarcoma virus RNA by avian myeloblastosis virus DNA polymerase and concurrent activation of the associated RNase H.禽成髓细胞瘤病毒DNA聚合酶对劳氏肉瘤病毒RNA进行广泛的体外转录，并同时激活相关的核糖核酸酶H。
J Virol. 1977 Sep;23(3):659-68. doi: 10.1128/JVI.23.3.659-668.1977.
6
In vitro transcription of theavian oncornavirus genome by the RNA-directed DNA polymerase: analysis of DNA transcripts synthesized in reconstructed enzymatic reactions.禽肿瘤病毒基因组由RNA指导的DNA聚合酶进行体外转录：对在重建酶促反应中合成的DNA转录本的分析
J Virol. 1977 Apr;22(1):86-96. doi: 10.1128/JVI.22.1.86-96.1977.
7
New procedure for the direct analysis of in vitro reverse transcription of Rous sarcoma virus RNA.劳氏肉瘤病毒RNA体外逆转录直接分析的新方法。
J Virol. 1977 Apr;22(1):118-29. doi: 10.1128/JVI.22.1.118-129.1977.

本文引用的文献

1
Mechanistic independence of avian myeloblastosis virus DNA polymerase and ribonuclease H.禽成髓细胞瘤病毒DNA聚合酶与核糖核酸酶H的机制独立性
J Virol. 1974 Dec;14(6):1494-502. doi: 10.1128/JVI.14.6.1494-1502.1974.
2
DNA polymerase of murine sarcoma-leukemia virus: lack of detectable RNase H and low activity with viral RNA and natural DNA templates.鼠肉瘤-白血病病毒的DNA聚合酶：未检测到核糖核酸酶H，对病毒RNA和天然DNA模板的活性较低。
J Virol. 1973 Dec;12(6):1512-21. doi: 10.1128/JVI.12.6.1512-1521.1973.
3
RNA-directed DNA polymerase of Rous sarcoma virus: initiation of synthesis with 70 S viral RNA as template.劳氏肉瘤病毒的RNA指导DNA聚合酶：以70S病毒RNA为模板起始合成
J Mol Biol. 1973 Sep 5;79(1):163-83. doi: 10.1016/0022-2836(73)90277-5.
4
RNA-directed DNA synthesis and RNA tumor viruses.RNA 指导的 DNA 合成与 RNA 肿瘤病毒
Adv Virus Res. 1972;17:129-86. doi: 10.1016/s0065-3527(08)60749-6.
5
Ribonucleases of Rous sarcoma virus.劳氏肉瘤病毒的核糖核酸酶
Virology. 1973 Feb;51(2):287-96. doi: 10.1016/0042-6822(73)90429-7.
6
Association of an endoribonuclease with the avian myeloblastosis virus deoxyribonucleic acid polymerase.一种核糖核酸酶与禽成髓细胞瘤病毒脱氧核糖核酸聚合酶的关联。
J Biol Chem. 1972 Nov 25;247(22):7282-7.
7
Degradation of DNA RNA hybrids by ribonuclease H and DNA polymerases of cellular and viral origin.细胞和病毒来源的核糖核酸酶H及DNA聚合酶对DNA-RNA杂交体的降解作用
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3360-4. doi: 10.1073/pnas.69.11.3360.
8
Ribonucleic acid directed deoxyribonucleic acid synthesis by the purified deoxyribonucleic acid polymerase of Rous sarcoma virus. Characterization of the enzymatic product.劳斯肉瘤病毒纯化的脱氧核糖核酸聚合酶催化的核糖核酸指导的脱氧核糖核酸合成。酶促产物的特性
Biochemistry. 1972 Jun 6;11(12):2343-51. doi: 10.1021/bi00762a021.
9
Purification and characterization of the deoxyribonucleic acid polymerase associated with Rous sarcoma virus.与劳氏肉瘤病毒相关的脱氧核糖核酸聚合酶的纯化与特性分析
Biochemistry. 1972 Jun 6;11(12):2334-42. doi: 10.1021/bi00762a020.
10
Association of viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA-DNA hybrids.病毒逆转录酶与一种降解RNA-DNA杂交体RNA部分的酶的关联。
Nat New Biol. 1971 Dec 22;234(51):240-3. doi: 10.1038/newbio234240a0.

劳氏肉瘤病毒的RNA指导DNA聚合酶对70S RNA的体外转录：核糖核酸酶H的影响缺失

In vitro transcription of 70S RNA by the RNA-directed DNA polymerase of Rouse sarcoma virus: lack of influence of RNase H.

作者信息

Collett M S, Faras A J

出版信息

J Virol. 1975 Jan;17(1):291-5. doi: 10.1128/JVI.17.1.291-295.1976.

DOI:10.1128/JVI.17.1.291-295.1976
PMID:54443
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC515415/
Abstract

The influence of Rous sarcoma virus (RSV)-associated RNase H on the in vitro synthesis of DNA by the RSV RNA-directed DNA polymerase was determined under conditions whereby RNase H activity was selectively inhibited with NaF. Not only were we unable to detect any effect on the size, structure, or genetic complixity of the DNA product synthesized in the absence of RNase H activity, but the displacement of DNA from the 70S RNA:DNA hybrid structures was also unaffected. The suitability of 70S RNA:DNA hybrid structures synthesized in vitro to serve as a substrate for RNase H is discussed.

摘要

在通过氟化钠选择性抑制核糖核酸酶H活性的条件下，测定了劳氏肉瘤病毒（RSV）相关核糖核酸酶H对RSV RNA指导的DNA聚合酶体外合成DNA的影响。我们不仅未能检测到在不存在核糖核酸酶H活性的情况下合成的DNA产物在大小、结构或遗传复杂性方面受到任何影响，而且从70S RNA:DNA杂交结构中置换DNA的过程也未受影响。本文讨论了体外合成的70S RNA:DNA杂交结构作为核糖核酸酶H底物的适用性。